MULTISPECIES: clavaminate synthase family protein [Pseudomonas].

LOCUS       WP_003082503             319 aa            linear   BCT 01-JAN-2025
ACCESSION   WP_003082503
VERSION     WP_003082503.1
KEYWORDS    RefSeq.
SOURCE      Pseudomonas
  ORGANISM  Pseudomonas
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Pseudomonadales; Pseudomonadaceae.
REFERENCE   1  (residues 1 to 319)
  AUTHORS   Islam,M.S., Leissing,T.M., Chowdhury,R., Hopkinson,R.J. and
            Schofield,C.J.
  TITLE     2-Oxoglutarate-Dependent Oxygenases
  JOURNAL   Annu Rev Biochem 87, 585-620 (2018)
   PUBMED   29494239
REFERENCE   2  (residues 1 to 319)
  AUTHORS   Schofield,C.J. and Zhang,Z.
  TITLE     Structural and mechanistic studies on 2-oxoglutarate-dependent
            oxygenases and related enzymes
  JOURNAL   Curr Opin Struct Biol 9 (6), 722-731 (1999)
   PUBMED   10607676
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 10083304
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Pseudomonas"
                     /db_xref="taxon:286"
     Protein         1..319
                     /product="clavaminate synthase family protein"
                     /EC_number="1.14.11.-"
                     /GO_function="GO:0005506 - iron ion binding [Evidence
                     IEA]"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /GO_function="GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /calculated_mol_wt=35794
     Region          28..285
                     /region_name="CAS_like"
                     /note="Clavaminic acid synthetase (CAS) -like; CAS is a
                     trifunctional Fe(II)/ 2-oxoglutarate (2OG) oxygenase
                     carrying out three reactions in the biosynthesis of
                     clavulanic acid, an inhibitor of class A serine
                     beta-lactamases. In general, Fe(II)-2OG oxygenases...;
                     cd00250"
                     /db_xref="CDD:238154"
     Site            order(94..95,126,128,133,182,279,281)
                     /site_type="other"
                     /note="substrate binding pocket [chemical binding]"
                     /db_xref="CDD:238154"
     Site            order(123,125,150,262,275)
                     /site_type="active"
                     /db_xref="CDD:238154"
     Site            order(123,125,262)
                     /site_type="other"
                     /note="iron coordination sites [ion binding]"
                     /db_xref="CDD:238154"
ORIGIN      
        1 mttihrrpap wrgnavfcas aafaagletl farllerrpa dsaeallrqr lhhelehgsg
       61 vllhdsallr glaedefqrv frafcqwlgr tvainrngey lkevrdlglr dardapqrgh
      121 ltsqelafhs dradltvlac wsparrggaf rirssadvla tleaanpawl pllgqpiphd
      181 lrdegdadya lvplltetpr tfvlryirkf nesvtrhgls lapqvrsmld gldeaierad
      241 gyaeldfskg mlvlvnnhtt lhartefsdd erqrrcllrc wlsseftrel pesflplfhq
      301 vaagslrggi rplaqeprp