MULTISPECIES: asparaginase [Klebsiella].

LOCUS       WP_002901285             339 aa            linear   BCT 06-MAR-2020
ACCESSION   WP_002901285
VERSION     WP_002901285.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella
  ORGANISM  Klebsiella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group.
REFERENCE   1  (residues 1 to 339)
  AUTHORS   Jerlstrom,P.G., Bezjak,D.A., Jennings,M.P. and Beacham,I.R.
  TITLE     Structure and expression in Escherichia coli K-12 of the
            L-asparaginase I-encoding ansA gene and its flanking regions
  JOURNAL   Gene 78 (1), 37-46 (1989)
   PUBMED   2670682
REFERENCE   2  (residues 1 to 339)
  AUTHORS   Spring,K.J., Jerlstrom,P.G., Burns,D.M. and Beacham,I.R.
  TITLE     L-asparaginase genes in Escherichia coli: isolation of mutants and
            characterization of the ansA gene and its protein product
  JOURNAL   J. Bacteriol. 166 (1), 135-142 (1986)
   PUBMED   3514575
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF006998.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK09461
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Klebsiella"
                     /db_xref="taxon:570"
     gene            1..339
                     /gene="ansA"
     Protein         1..339
                     /product="asparaginase"
                     /GO_function="GO:0004067 - asparaginase activity [Evidence
                     IEA]"
                     /GO_process="GO:0006520 - cellular amino acid metabolic
                     process [Evidence IEA]"
                     /calculated_mol_wt=36907
     Region          1..335
                     /region_name="ansA"
                     /note="cytoplasmic asparaginase I; Provisional; PRK09461"
                     /db_xref="CDD:181876"
     Site            order(13..14,60..61,90..92,117,163)
                     /site_type="active"
                     /db_xref="CDD:199207"
     Site            order(14,23..25,56,59..65,67..68,92..93,96,118..119,
                     163..168,210,212..221,223..224,227,230..231,240,242..244,
                     248,272,275..279,283,302,306,310)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:199207"
ORIGIN      
        1 mqkksiyvay tggtigmqrs ehgyvpvsgh lqrqlalmpe fhrpempdft iheyhplmds
       61 sdmtpedwqh iaddirshye eydgfvilhg tdtmaftasa lsfmlenlgk pvivtgsqip
      121 laelrsdgqi nllnalyvaa nypinevalf fnnrlfrgnr ttkahadgfd afaspnlapl
      181 leagihirrl gtppapqgsg dlivhpitpq pigvvtiypg isadvvrnfl rqpvkalilr
      241 sygvgnapqn gefiqvlaea sqrgivvvnl tqcmsgkvnm ggyatgnala qagvisgfdm
      301 tveatltklh yllsqqldvd airaamqqnl rgeltpdea