MULTISPECIES: tRNA (N6-isopentenyl

LOCUS       WP_002894730             474 aa            linear   BCT 20-NOV-2023
            adenosine(37)-C2)-methylthiotransferase MiaB [Klebsiella].
ACCESSION   WP_002894730
VERSION     WP_002894730.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella
  ORGANISM  Klebsiella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group.
REFERENCE   1  (residues 1 to 474)
  AUTHORS   Pierrel,F., Hernandez,H.L., Johnson,M.K., Fontecave,M. and Atta,M.
  TITLE     MiaB protein from Thermotoga maritima. Characterization of an
            extremely thermophilic tRNA-methylthiotransferase
  JOURNAL   J Biol Chem 278 (32), 29515-29524 (2003)
   PUBMED   12766153
REFERENCE   2  (residues 1 to 474)
  AUTHORS   Pierrel,F., Bjork,G.R., Fontecave,M. and Atta,M.
  TITLE     Enzymatic modification of tRNAs: MiaB is an iron-sulfur protein
  JOURNAL   J Biol Chem 277 (16), 13367-13370 (2002)
   PUBMED   11882645
REFERENCE   3  (residues 1 to 474)
  AUTHORS   Anantharaman,V., Koonin,E.V. and Aravind,L.
  TITLE     TRAM, a predicted RNA-binding domain, common to tRNA uracil
            methylation and adenine thiolation enzymes
  JOURNAL   FEMS Microbiol Lett 197 (2), 215-221 (2001)
   PUBMED   11313137
REFERENCE   4  (residues 1 to 474)
  AUTHORS   Esberg,B., Leung,H.C., Tsui,H.C., Bjork,G.R. and Winkler,M.E.
  TITLE     Identification of the miaB gene, involved in methylthiolation of
            isopentenylated A37 derivatives in the tRNA of Salmonella
            typhimurium and Escherichia coli
  JOURNAL   J Bacteriol 181 (23), 7256-7265 (1999)
   PUBMED   10572129
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR01574.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..474
                     /organism="Klebsiella"
                     /db_xref="taxon:570"
     gene            1..474
                     /gene="miaB"
     Protein         1..474
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /EC_number="2.8.4.3"
                     /GO_function="GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA]"
                     /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster
                     binding [Evidence IEA]"
                     /GO_function="GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence
                     IEA]"
                     /calculated_mol_wt=53420
     Region          4..440
                     /region_name="miaB-methiolase"
                     /note="tRNA-N(6)-(isopentenyl)adenosine-37 thiotransferase
                     enzyme MiaB; TIGR01574"
                     /db_xref="CDD:273700"
ORIGIN      
        1 mtkklhiktw gcqmneydss kmadlldath gyqltevaee advlllntcs irekaqekvf
       61 hqlgrwkllk eknpdliigv ggcvasqegd hirqrahyvd iifgpqtlhr lpeminsvrg
      121 nrspvvdisf peiekfdrlp epraegptaf vsimegcnky ctycvvpytr geevsrpcdd
      181 ilfeiaqlaa qgvrevnllg qnvnawrgen ydgttgsfad llrlvaaidg idrirfttsh
      241 pieftddiid vyrdtpelvs flhlpvqsgs drvlnlmgrt htaleykaii rklrearpdi
      301 qissdfivgf pgettedfek tmkliadvnf dmsysfifsa rpgtpaadmv ddvpeadkkq
      361 rlyilqerin qqamawsrrm lgtvqrilve gtsrknimel sgrtennrvv nfegtpdlvg
      421 kfvdveivdv ytnslrgkiv rteaemglri aespesviar trkendlgvg iyqp