MULTISPECIES: tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase MiaE

LOCUS       WP_002886948             254 aa            linear   BCT 10-JAN-2025
            [Klebsiella].
ACCESSION   WP_002886948
VERSION     WP_002886948.1
KEYWORDS    RefSeq.
SOURCE      Klebsiella
  ORGANISM  Klebsiella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Klebsiella/Raoultella group.
REFERENCE   1  (residues 1 to 254)
  AUTHORS   Mathevon,C., Pierrel,F., Oddou,J.L., Garcia-Serres,R., Blondin,G.,
            Latour,J.M., Menage,S., Gambarelli,S., Fontecave,M. and Atta,M.
  TITLE     tRNA-modifying MiaE protein from Salmonella typhimurium is a
            nonheme diiron monooxygenase
  JOURNAL   Proc Natl Acad Sci U S A 104 (33), 13295-13300 (2007)
   PUBMED   17679698
REFERENCE   2  (residues 1 to 254)
  AUTHORS   Persson,B.C. and Bjork,G.R.
  TITLE     Isolation of the gene (miaE) encoding the hydroxylase involved in
            the synthesis of 2-methylthio-cis-ribozeatin in tRNA of Salmonella
            typhimurium and characterization of mutants
  JOURNAL   J Bacteriol 175 (24), 7776-7785 (1993)
   PUBMED   8253666
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF047790.1
            Evidence Source    :: NCBIFAM
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..254
                     /organism="Klebsiella"
                     /db_xref="taxon:570"
     gene            1..254
                     /gene="miaE"
     Protein         1..254
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /EC_number="1.14.99.69"
                     /GO_function="GO:0004497 - monooxygenase activity
                     [Evidence IEA]"
                     /GO_function="GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence
                     IEA]"
                     /calculated_mol_wt=29259
     Region          5..248
                     /region_name="Ferritin_like"
                     /note="Ferritin-like superfamily of diiron-containing
                     four-helix-bundle proteins; cl00264"
                     /db_xref="CDD:469698"
     Site            order(43,121,124,174,203,206)
                     /site_type="other"
                     /note="dinuclear metal binding motif [ion binding]"
                     /db_xref="CDD:153097"
ORIGIN      
        1 mdypqilspi isflhcptpq awidearkpe nlpllltdhm vcelkaaqna mllvrryvad
       61 kadadellac lkpyedftyr rgpepdfvtl hkrinksamp qtddpwgrql ldsmillike
      121 elhhfwqvre mmlardipyv kitasnyarg lrrevrshep vmlidklicg ayiearscer
      181 faalapwldd dlqkfylsll rsearhyqdy ldlaqkiage diservrqlg eaeaalilrp
      241 eaefrfhsgv pvaa