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NADPH-dependent aldehyde reductase Ahr [Escherichia coli].


LOCUS       WP_001301967             339 aa            linear   BCT 14-JAN-2025
ACCESSION   WP_001301967
VERSION     WP_001301967.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 339)
  AUTHORS   Pick,A., Ruhmann,B., Schmid,J. and Sieber,V.
  TITLE     Novel CAD-like enzymes from Escherichia coli K-12 as additional
            tools in chemical production
  JOURNAL   Appl Microbiol Biotechnol 97 (13), 5815-5824 (2013)
   PUBMED   23093176
REFERENCE   2  (residues 1 to 339)
  AUTHORS   Akhtar,M.K., Turner,N.J. and Jones,P.R.
  TITLE     Carboxylic acid reductase is a versatile enzyme for the conversion
            of fatty acids into fuels and chemical commodities
  JOURNAL   Proc Natl Acad Sci U S A 110 (1), 87-92 (2013)
   PUBMED   23248280
REFERENCE   3  (residues 1 to 339)
  AUTHORS   Rodriguez,G.M. and Atsumi,S.
  TITLE     Isobutyraldehyde production from Escherichia coli by removing
            aldehyde reductase activity
  JOURNAL   Microb Cell Fact 11, 90 (2012)
   PUBMED   22731523
  REMARK    Publication Status: Online-Only
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF047908.1
            Evidence Source    :: NCBIFAM
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..339
                     /gene="ahr"
     Protein         1..339
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /EC_number="1.1.1.2"
                     /GO_function="GO:0008106 - alcohol dehydrogenase (NADP+)
                     activity [Evidence IEA]"
                     /GO_function="GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /GO_process="GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /calculated_mol_wt=36397
     Region          5..336
                     /region_name="CAD1"
                     /note="Cinnamyl alcohol dehydrogenases (CAD); cd05283"
                     /db_xref="CDD:176186"
     Site            order(41..43,46,152,156,176..181,199..200,204,219,
                     238..239,241,261..262,285..287)
                     /site_type="other"
                     /note="putative NAD(P) binding site [chemical binding]"
                     /db_xref="CDD:176186"
     Site            order(41,43,63,91,152,287)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:176186"
     Site            order(41,63,152)
                     /site_type="other"
                     /note="catalytic Zn binding site [ion binding]"
                     /db_xref="CDD:176186"
     Site            order(96,99,102,110)
                     /site_type="other"
                     /note="structural Zn binding site [ion binding]"
                     /db_xref="CDD:176186"
     Site            order(109,159,163,255,260..262,264,273,275..276,279..286)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:176186"
ORIGIN      
        1 msmiksyaak eaggelevye ydpgelrpqd vevqvdycgi chsdlsmidn ewgfsqyplv
       61 aghevigrvv algsaaqdkg lqvgqrvgig wtarscghcd acisgnqinc eqgavptimn
      121 rggfaeklra dwqwviplpe nidiesagpl lcggitvfkp llmhhitats rvgvigiggl
      181 ghiaikllha mgcevtafss npakeqevla mgadkvvnsr dpqalkalsg qfdliintvn
      241 vsldwqpyfe altyggnfht vgavltplpv paftliagdr svsgsatgtp yelrklmrfa
      301 arskvaptte lfpmskinda ikhvrdgkar yrvvlkadf