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LOCUS WP_001301861 368 aa linear BCT 27-DEC-2023 ACCESSION WP_001301861 VERSION WP_001301861.1 KEYWORDS RefSeq. SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (residues 1 to 368) AUTHORS Numata,T., Ikeuchi,Y., Fukai,S., Suzuki,T. and Nureki,O. TITLE Snapshots of tRNA sulphuration via an adenylated intermediate JOURNAL Nature 442 (7101), 419-424 (2006) PUBMED 16871210 REFERENCE 2 (residues 1 to 368) AUTHORS Kambampati,R. and Lauhon,C.T. TITLE MnmA and IscS are required for in vitro 2-thiouridine biosynthesis in Escherichia coli JOURNAL Biochemistry 42 (4), 1109-1117 (2003) PUBMED 12549933 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: HMM Evidence Accession :: TIGR00420.1 Evidence Source :: JCVI ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..368 /organism="Escherichia coli" /db_xref="taxon:562" gene 1..368 /gene="mnmA" /gene_synonym="trmU" Protein 1..368 /product="tRNA 2-thiouridine(34) synthase MnmA" /EC_number="2.8.1.13" /GO_component="GO:0005737 - cytoplasm [Evidence IEA]" /GO_function="GO:0004808 - tRNA (5-methylaminomethyl-2-thiouridylate)(34)- methyltransferase activity [Evidence IEA]" /GO_function="GO:0016783 - sulfurtransferase activity [Evidence IEA]" /GO_process="GO:0002143 - tRNA wobble position uridine thiolation [Evidence IEA]" /GO_process="GO:0006396 - RNA processing [Evidence IEA]" /calculated_mol_wt=40856 Region 6..365 /region_name="MnmA" /note="tRNA U34 2-thiouridine synthase MnmA/TrmU, contains the PP-loop ATPase domain [Translation, ribosomal structure and biogenesis]; COG0482" /db_xref="CDD:440250" ORIGIN 1 msetakkviv gmsggvdssv sawllqqqgy qveglfmknw eeddgeeyct aaadladaqa 61 vcdklgielh tvnfaaeywd nvfelflaey kagrtpnpdi lcnkeikfka flefaaedlg 121 adyiatghyv rradvdgksr llrgldsnkd qsyflytlsh eqiaqslfpv gelekpqvrk 181 iaedlglvta kkkdstgicf igerkfrefl grylpaqpgk iitvdgdeig ehqglmyhtl 241 gqrkglgigg tkegteepwy vvdkdvenni livaqghehp rlmsvgliaq qlhwvdrepf 301 tgtmrctvkt ryrqtdipct vkaldddrie vifdepvaav tpgqsavfyn gevclgggii 361 eqrlplpi