MULTISPECIES: GDP-L-fucose synthase [Escherichia].

LOCUS       WP_001301811             321 aa            linear   BCT 13-JAN-2025
ACCESSION   WP_001301811
VERSION     WP_001301811.1
KEYWORDS    RefSeq.
SOURCE      Escherichia
  ORGANISM  Escherichia
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae.
REFERENCE   1  (residues 1 to 321)
  AUTHORS   Rosano,C., Bisso,A., Izzo,G., Tonetti,M., Sturla,L., De Flora,A.
            and Bolognesi,M.
  TITLE     Probing the catalytic mechanism of GDP-4-keto-6-deoxy-d-mannose
            Epimerase/Reductase by kinetic and crystallographic
            characterization of site-specific mutants
  JOURNAL   J Mol Biol 303 (1), 77-91 (2000)
   PUBMED   11021971
REFERENCE   2  (residues 1 to 321)
  AUTHORS   Menon,S., Stahl,M., Kumar,R., Xu,G.Y. and Sullivan,F.
  TITLE     Stereochemical course and steady state mechanism of the reaction
            catalyzed by the GDP-fucose synthetase from Escherichia coli
  JOURNAL   J Biol Chem 274 (38), 26743-26750 (1999)
   PUBMED   10480878
REFERENCE   3  (residues 1 to 321)
  AUTHORS   Andrianopoulos,K., Wang,L. and Reeves,P.R.
  TITLE     Identification of the fucose synthetase gene in the colanic acid
            gene cluster of Escherichia coli K-12
  JOURNAL   J Bacteriol 180 (4), 998-1001 (1998)
   PUBMED   9473059
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF047922.1
            Evidence Source    :: NCBIFAM
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Escherichia"
                     /db_xref="taxon:561"
     gene            1..321
                     /gene="fcl"
                     /gene_synonym="wcaG; yefB"
                     /GO_function="GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
     Protein         1..321
                     /product="GDP-L-fucose synthase"
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /GO_function="GO:0050577 - GDP-L-fucose synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /calculated_mol_wt=36138
     Region          5..313
                     /region_name="GDP_FS_SDR_e"
                     /note="GDP-fucose synthetase, extended (e) SDRs; cd05239"
                     /db_xref="CDD:187550"
     Site            order(10,12..15,36,39..42,62..64,66,86,105..107,136,140,
                     163..164,166)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:187550"
     Site            order(83,107,136,140)
                     /site_type="active"
                     /db_xref="CDD:187550"
     Site            order(107,136,165,184,201,209)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187550"
ORIGIN      
        1 mnkqrifiag hqgmvgsait rrlkqrddve lvlrtrdeln lldssavldf fssqkidqvy
       61 laaakvggil anssypadfi yenimieanv ihaahknnvn kllflgssci ypklahqpim
      121 edellqgkle ptnepyaiak iagiklcesy nrqfgrdyrs vmptnlygpn dnfhpsnshv
      181 ipallrrfhd avennspnvv vwgsgtpkre flhvddmasa siyvmempyd iwqkntkvml
      241 shinigtgid cticelaeti akvvgykghi tfdttkpdga prklldvtll hqlgwnhkit
      301 lhkglentyn wflenqlqyr g