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LOCUS WP_001301445 729 aa linear BCT 20-MAR-2023 ACCESSION WP_001301445 VERSION WP_001301445.1 KEYWORDS RefSeq. SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (residues 1 to 729) AUTHORS Tchigvintsev,A., Xu,X., Singer,A., Chang,C., Brown,G., Proudfoot,M., Cui,H., Flick,R., Anderson,W.F., Joachimiak,A., Galperin,M.Y., Savchenko,A. and Yakunin,A.F. TITLE Structural insight into the mechanism of c-di-GMP hydrolysis by EAL domain phosphodiesterases JOURNAL J Mol Biol 402 (3), 524-538 (2010) PUBMED 20691189 REFERENCE 2 (residues 1 to 729) AUTHORS Schmidt,A.J., Ryjenkov,D.A. and Gomelsky,M. TITLE The ubiquitous protein domain EAL is a cyclic diguanylate-specific phosphodiesterase: enzymatically active and inactive EAL domains JOURNAL J Bacteriol 187 (14), 4774-4781 (2005) PUBMED 15995192 REFERENCE 3 (residues 1 to 729) AUTHORS Nikolskaya,A.N., Mulkidjanian,A.Y., Beech,I.B. and Galperin,M.Y. TITLE MASE1 and MASE2: two novel integral membrane sensory domains JOURNAL J Mol Microbiol Biotechnol 5 (1), 11-16 (2003) PUBMED 12673057 REFERENCE 4 (residues 1 to 729) AUTHORS Pei,J. and Grishin,N.V. TITLE GGDEF domain is homologous to adenylyl cyclase JOURNAL Proteins 42 (2), 210-216 (2001) PUBMED 11119645 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: Conserved Domain (CDD) Evidence Accession :: Domain architecture ID 11156636 Evidence Source :: NCBI SPARCLE ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..729 /organism="Escherichia coli" /db_xref="taxon:562" Protein 1..729 /product="sensor domain-containing phosphodiesterase" /EC_number="3.1.4.52" /GO_function="GO:0071111 - cyclic-guanylate-specific phosphodiesterase activity [Evidence IEA]" /calculated_mol_wt=83439 Region 15..311 /region_name="MASE1" /note="pfam05231" /db_xref="CDD:428383" Region 169..729 /region_name="EAL" /note="EAL domain, c-di-GMP-specific phosphodiesterase class I (or its enzymatically inactive variant) [Signal transduction mechanisms]; COG2200" /db_xref="CDD:441802" Region 493..729 /region_name="EAL" /note="Putative diguanylate phosphodiesterase; smart00052" /db_xref="CDD:214491" ORIGIN 1 mfvehnlikn ikiftlaftl tvvliqlsrf isplaiihss yiflawmplc vmlsilfifg 61 wrgvvpvlcg mfctnlwnfh lsflqtavml gsqtfvvlca cailrwqlgt rwrygltsry 121 vwqrlfwlgl vtpigikcsm ylvgsffdfp lkistffgda daiftvvdll slftavliyn 181 mlfyyltrmi vsphfaqilw rrdiapslgk ekraftlswl aalsvlllll ctpyendfia 241 gylvpvffii ftlgvgklry pflnltwavs tlcllnynqn flqgveteys lafilavlis 301 fsvcllymvr iyhrsewlnr rwhlqaltdp ltllpnfral eqapeqeagk sfcclridnl 361 efmsrhyglm mrvhcirsiy rtllplmqen eklyqlpgse lllvlsgpet egrlqhmvni 421 lnsrqihwnn tgldmgygaa wgrfdgnqet lqpllgqlsw laeqscahhh vlaldsreem 481 vsgqttkqvl llntirtald qgdlllyaqp irnkegegyd evlarlkydg gimtpdkflp 541 liaqfnlsar fdlqvlesll kwlathpcdk kgprfsvnlm pltllqknia griirlfkry 601 hispqavile iteeqafsna essmynieql hkfgfriaid dfgtgyanye rlkrlqadii 661 kidgvfvkdi vtntldamiv rsitdlakak slsvvaefve tqqqqallhk lgvqylqgyl 721 igrpqplad