Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]

N-acetylmuramoyl-L-alanine amidase AmiD [Escherichia coli].


LOCUS       WP_001255150             276 aa            linear   BCT 10-FEB-2020
ACCESSION   WP_001255150
VERSION     WP_001255150.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 276)
  AUTHORS   Kerff,F., Petrella,S., Mercier,F., Sauvage,E., Herman,R.,
            Pennartz,A., Zervosen,A., Luxen,A., Frere,J.M., Joris,B. and
            Charlier,P.
  TITLE     Specific structural features of the N-acetylmuramoyl-L-alanine
            amidase AmiD from Escherichia coli and mechanistic implications for
            enzymes of this family
  JOURNAL   J. Mol. Biol. 397 (1), 249-259 (2010)
   PUBMED   20036252
REFERENCE   2  (residues 1 to 276)
  AUTHORS   Uehara,T. and Park,J.T.
  TITLE     An anhydro-N-acetylmuramyl-L-alanine amidase with broad specificity
            tethered to the outer membrane of Escherichia coli
  JOURNAL   J. Bacteriol. 189 (15), 5634-5641 (2007)
   PUBMED   17526703
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: BlastRule
            Evidence Accession :: NBR011118
            Evidence Source    :: NCBI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..276
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..276
                     /gene="amiD"
     Protein         1..276
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /EC_number="3.5.1.28"
                     /calculated_mol_wt=30935
     Region          43..189
                     /region_name="AmpD"
                     /note="N-acetyl-anhydromuramyl-L-alanine amidase AmpD
                     [Cell wall/membrane/envelope biogenesis]; COG3023"
                     /db_xref="CDD:442259"
ORIGIN      
        1 mrrvfwliav alllagcage kgivekegyq ldtrrqaqaa yprikvlvih ytaddfdssl
       61 atltdkqvss hylvpavppr yngkpriwql vpeqelawha gisawrgatr lndtsigiel
      121 enrgwqksag vkyfapfepa qiqaliplak diiaryhikp envvahadia pqrkddpgpl
      181 fpwqqlaqqg igawpdaqrv nfylagraph tpvdtaslle llarygydvk pdmtpreqrr
      241 vimafqmhfr ptlyngeada etqaiaeall ekygqd