RNA-guided endonuclease InsQ/TnpB family protein [Escherichia

LOCUS       WP_001250878             382 aa            linear   BCT 23-DEC-2024
            coli].
ACCESSION   WP_001250878
VERSION     WP_001250878.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 382)
  AUTHORS   Karvelis,T., Druteika,G., Bigelyte,G., Budre,K., Zedaveinyte,R.,
            Silanskas,A., Kazlauskas,D., Venclovas,C. and Siksnys,V.
  TITLE     Transposon-associated TnpB is a programmable RNA-guided DNA
            endonuclease
  JOURNAL   Nature 599 (7886), 692-696 (2021)
   PUBMED   34619744
REFERENCE   2  (residues 1 to 382)
  AUTHORS   Siguier,P., Gourbeyre,E. and Chandler,M.
  TITLE     Bacterial insertion sequences: their genomic impact and diversity
  JOURNAL   FEMS Microbiol Rev 38 (5), 865-891 (2014)
   PUBMED   24499397
REFERENCE   3  (residues 1 to 382)
  AUTHORS   Rice,P.A. and Baker,T.A.
  TITLE     Comparative architecture of transposase and integrase complexes
  JOURNAL   Nat Struct Biol 8 (4), 302-307 (2001)
   PUBMED   11276247
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 11430747
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..382
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     Protein         1..382
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /EC_number="3.1.21.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence
                     IEA]"
                     /GO_process="GO:0032196 - transposition [Evidence IEA]"
                     /calculated_mol_wt=43047
     Region          1..372
                     /region_name="InsQ"
                     /note="Transposase [Mobilome: prophages, transposons];
                     COG0675"
                     /db_xref="CDD:440439"
ORIGIN      
        1 mrrfagacrf vfnralalqn enheagnkyi pygkmaswlv ewknatetqw lkdapsqplq
       61 qslkdleray knffrkraaf prfkkrgqnd afrypqgvkl dqensriflp klgwmryrns
      121 rqvtgvvknv tasqscgkwy isiqtenevs tpvhpsalmv gldagvakla tlsdgtvfgp
      181 vnsfqknqkt larlqrqlsr kvkfsnnwqk qkrkiqrlhs cianicrdyl hkvtttvskn
      241 hamiviedlk vsnmsksaag tvsqpgrnvr aksglnrsil dqgwyemrrq leykqlwrgg
      301 qvlavppayt sqrcaccght akenrlsqsk frcqacgyta nadvngarni laaghavlac
      361 gemvqsgrpl kqeptemiqa ta