MULTISPECIES: hydroxyethylthiazole kinase [Escherichia].

LOCUS       WP_001195634             262 aa            linear   BCT 17-JAN-2025
ACCESSION   WP_001195634
VERSION     WP_001195634.1
KEYWORDS    RefSeq.
SOURCE      Escherichia
  ORGANISM  Escherichia
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae.
REFERENCE   1  (residues 1 to 262)
  AUTHORS   Drebes,J., Kunz,M., Windshugel,B., Kikhney,A.G., Muller,I.B.,
            Eberle,R.J., Oberthur,D., Cang,H., Svergun,D.I., Perbandt,M.,
            Betzel,C. and Wrenger,C.
  TITLE     Structure of ThiM from Vitamin B1 biosynthetic pathway of
            Staphylococcus aureus - Insights into a novel pro-drug approach
            addressing MRSA infections
  JOURNAL   Sci Rep 6, 22871 (2016)
   PUBMED   26960569
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 262)
  AUTHORS   Campobasso,N., Mathews,I.I., Begley,T.P. and Ealick,S.E.
  TITLE     Crystal structure of 4-methyl-5-beta-hydroxyethylthiazole kinase
            from Bacillus subtilis at 1.5 A resolution
  JOURNAL   Biochemistry 39 (27), 7868-7877 (2000)
   PUBMED   10891066
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF006830.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK09355
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..262
                     /organism="Escherichia"
                     /db_xref="taxon:561"
     gene            1..262
                     /gene="thiM"
     Protein         1..262
                     /product="hydroxyethylthiazole kinase"
                     /EC_number="2.7.1.50"
                     /GO_function="GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=27224
     Region          15..259
                     /region_name="HK"
                     /note="Hydroxyethylthiazole kinase family; pfam02110"
                     /db_xref="CDD:396609"
     Site            order(30,32,38,42,48,50,70,72..73)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238575"
     Site            order(31..36,38..39,42,50..53,55..56,59..60,72..75,
                     108..109,194..197,243..244,246..248,250..251,254)
                     /site_type="other"
                     /note="multimerization interface [polypeptide binding]"
                     /db_xref="CDD:238575"
     Site            order(99,125,127,130,171,173,175,193,199,201)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:238575"
ORIGIN      
        1 mqvdllssaq sahtlhlfhq hsplvhcmtn dvvqtftant llalgaspam vieteeasqf
       61 aaiasallin vgtltqpraq amraaveqak ssqtpwtldp vavgaldyrr hfchellsfk
      121 paairgnase imalagvang grgvdttdaa vnaipaaqtl aretgaivvv tgevdyvtdg
      181 hravgihggd plmtkvvgtg calsavvaac calpgdmlen vasachwmkq ageravarse
      241 gpgsfvphfl dalwqltqev qa