methylenetetrahydrofolate reductase [Escherichia coli].

LOCUS       WP_001008791             296 aa            linear   BCT 10-DEC-2019
ACCESSION   WP_001008791
VERSION     WP_001008791.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 296)
  AUTHORS   Trimmer,E.E., Ballou,D.P., Galloway,L.J., Scannell,S.A.,
            Brinker,D.R. and Casas,K.R.
  TITLE     Aspartate 120 of Escherichia coli methylenetetrahydrofolate
            reductase: evidence for major roles in folate binding and catalysis
            and a minor role in flavin reactivity
  JOURNAL   Biochemistry 44 (18), 6809-6822 (2005)
   PUBMED   15865426
REFERENCE   2  (residues 1 to 296)
  AUTHORS   Haller,T., Buckel,T., Retey,J. and Gerlt,J.A.
  TITLE     Discovering new enzymes and metabolic pathways: conversion of
            succinate to propionate by Escherichia coli
  JOURNAL   Biochemistry 39 (16), 4622-4629 (2000)
   PUBMED   10769117
REFERENCE   3  (residues 1 to 296)
  AUTHORS   Guenther,B.D., Sheppard,C.A., Tran,P., Rozen,R., Matthews,R.G. and
            Ludwig,M.L.
  TITLE     The structure and properties of methylenetetrahydrofolate reductase
            from Escherichia coli suggest how folate ameliorates human
            hyperhomocysteinemia
  JOURNAL   Nat. Struct. Biol. 6 (4), 359-365 (1999)
   PUBMED   10201405
REFERENCE   4  (residues 1 to 296)
  AUTHORS   Saint-Girons,I., Duchange,N., Zakin,M.M., Park,I., Margarita,D.,
            Ferrara,P. and Cohen,G.N.
  TITLE     Nucleotide sequence of metF, the E. coli structural gene for 5-10
            methylene tetrahydrofolate reductase and of its control region
  JOURNAL   Nucleic Acids Res. 11 (19), 6723-6732 (1983)
   PUBMED   6356036
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF006950.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK09432
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..296
                     /gene="metF"
     Protein         1..296
                     /product="methylenetetrahydrofolate reductase"
                     /EC_number="1.5.1.20"
                     /GO_function="GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA]"
                     /GO_process="GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=32999
     Region          1..296
                     /region_name="metF"
                     /note="methylenetetrahydrofolate reductase; PRK09432"
                     /db_xref="CDD:181852"
     Site            order(60,88,117..119,131..132,150,152,156,158,165,168,
                     171..172,181,183,275)
                     /site_type="other"
                     /note="FAD binding site [chemical binding]"
                     /db_xref="CDD:238299"
ORIGIN      
        1 mnffhasqrd alnqslaevq gqinvsfeff pprtsemeqt lwnsidrlss lkpkfvsvty
       61 gansgerdrt hsiikgikdr tgleaaphlt cidatpdelr tiardywnng irhivalrgd
      121 lppgsgkpem yasdlvtllk evadfdisva aypevhpeak saqadllnlk rkvdaganra
      181 itqfffdves ylrfrdrcvs agidveiipg ilpvsnfkqa kkfadmtnvr ipawmaqmfd
      241 gldddaetrk lvganiamdm vkilsregvk dfhfytlnra emsyaichtl gvrpgl