Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]

MULTISPECIES: 23S rRNA pseudouridine(2604) synthase RluF


LOCUS       WP_000936361             290 aa            linear   BCT 25-APR-2022
            [Enterobacteriaceae].
ACCESSION   WP_000936361
VERSION     WP_000936361.1
KEYWORDS    RefSeq.
SOURCE      Enterobacteriaceae
  ORGANISM  Enterobacteriaceae
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales.
REFERENCE   1  (residues 1 to 290)
  AUTHORS   Addepalli,B. and Limbach,P.A.
  TITLE     Pseudouridine in the Anticodon of Escherichia coli tRNATyr(QPsiA)
            Is Catalyzed by the Dual Specificity Enzyme RluF
  JOURNAL   J Biol Chem 291 (42), 22327-22337 (2016)
   PUBMED   27551044
REFERENCE   2  (residues 1 to 290)
  AUTHORS   Del Campo,M., Kaya,Y. and Ofengand,J.
  TITLE     Identification and site of action of the remaining four putative
            pseudouridine synthases in Escherichia coli
  JOURNAL   RNA 7 (11), 1603-1615 (2001)
   PUBMED   11720289
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF007784.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK10475
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterobacteriaceae"
                     /db_xref="taxon:543"
     gene            1..290
                     /gene="rluF"
     Protein         1..290
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /EC_number="5.4.99.21"
                     /GO_function="GO:0003723 - RNA binding [Evidence IEA]"
                     /GO_function="GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0001522 - pseudouridine synthesis
                     [Evidence IEA]"
                     /calculated_mol_wt=32273
     Region          1..290
                     /region_name="PRK10475"
                     /note="23S rRNA pseudouridine(2604) synthase RluF"
                     /db_xref="CDD:236698"
ORIGIN      
        1 mlpdssvrln kyisesgics rreadryieq gnvflngkra tigdqvkpgd vvkvngqlie
       61 preaedlvli alnkpvgivs ttedgerdni vdfvnhskrv fpigrldkds qglifltnhg
      121 dlvnkilrag ndhekeylvt vdkpitdefi rgmgagvpil gtvtkkckvk keapfvfrit
      181 lvqglnrqir rmcehfgyev kklertrimn vslsgiplge wrdltddeli dlfklienss
      241 seakpkakak pktagikrpv vkmektaekg grpasngkrf tspgrkkkgr