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LOCUS WP_000932342 157 aa linear BCT 21-JAN-2024 (uridine(34)/cytosine(34)/5-carboxymethylaminomethyluridine(34)-2'- O)-methyltransferase TrmL [Enterobacteriaceae]. ACCESSION WP_000932342 VERSION WP_000932342.1 KEYWORDS RefSeq. SOURCE Enterobacteriaceae ORGANISM Enterobacteriaceae Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales. REFERENCE 1 (residues 1 to 157) AUTHORS Purta,E., van Vliet,F., Tkaczuk,K.L., Dunin-Horkawicz,S., Mori,H., Droogmans,L. and Bujnicki,J.M. TITLE The yfhQ gene of Escherichia coli encodes a tRNA:Cm32/Um32 methyltransferase JOURNAL BMC Mol. Biol. 7, 23 (2006) PUBMED 16848900 REFERENCE 2 (residues 1 to 157) AUTHORS Anantharaman,V., Koonin,E.V. and Aravind,L. TITLE SPOUT: a class of methyltransferases that includes spoU and trmD RNA methylase superfamilies, and novel superfamilies of predicted prokaryotic RNA methylases JOURNAL J. Mol. Microbiol. Biotechnol. 4 (1), 71-75 (2002) PUBMED 11763972 REFERENCE 3 (residues 1 to 157) AUTHORS Koonin,E.V. and Rudd,K.E. TITLE SpoU protein of Escherichia coli belongs to a new family of putative rRNA methylases JOURNAL Nucleic Acids Res. 21 (23), 5519 (1993) PUBMED 8265370 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: HMM Evidence Accession :: NF007683.0 Evidence Source :: NCBI Protein Cluster (PRK) Source Identifier :: PRK10358 ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..157 /organism="Enterobacteriaceae" /db_xref="taxon:543" gene 1..157 /gene="trmL" Protein 1..157 /product="tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL" /EC_number="2.1.1.-" /EC_number="2.1.1.207" /GO_function="GO:0008173 - RNA methyltransferase activity [Evidence IEA]" /GO_process="GO:0001510 - RNA methylation [Evidence IEA]" /calculated_mol_wt=17620 Region 1..157 /region_name="PRK10358" /note="tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL" /db_xref="CDD:182406" Site order(17,20..21,23..24,26,50,53,86..87,121..125,129..132, 134..135,138..139,142..143,145..146) /site_type="other" /note="dimer interface [polypeptide binding]" /db_xref="CDD:349967" Site order(78..80,99..102,104..106,120..122,124,129..131,133, 136) /site_type="other" /note="SAM binding site [chemical binding]" /db_xref="CDD:349967" ORIGIN 1 mlnivlyepe ippntgniir lcantgfrlh iiepmgfawd dkrlrragld yheftavtrh 61 hdfrafleae npqrlfaltt kgtpahsavn yqdgdylmfg petrglpasi ldalpaeqki 121 ripmvpdsrs mnlsnavsvv vyeawrqlgy pgallrd