MULTISPECIES: RNA-guided endonuclease InsQ/TnpB family protein

LOCUS       WP_000826416             402 aa            linear   BCT 24-DEC-2024
            [Enterobacteriaceae].
ACCESSION   WP_000826416
VERSION     WP_000826416.1
KEYWORDS    RefSeq.
SOURCE      Enterobacteriaceae
  ORGANISM  Enterobacteriaceae
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales.
REFERENCE   1  (residues 1 to 402)
  AUTHORS   Karvelis,T., Druteika,G., Bigelyte,G., Budre,K., Zedaveinyte,R.,
            Silanskas,A., Kazlauskas,D., Venclovas,C. and Siksnys,V.
  TITLE     Transposon-associated TnpB is a programmable RNA-guided DNA
            endonuclease
  JOURNAL   Nature 599 (7886), 692-696 (2021)
   PUBMED   34619744
REFERENCE   2  (residues 1 to 402)
  AUTHORS   Siguier,P., Gourbeyre,E. and Chandler,M.
  TITLE     Bacterial insertion sequences: their genomic impact and diversity
  JOURNAL   FEMS Microbiol Rev 38 (5), 865-891 (2014)
   PUBMED   24499397
REFERENCE   3  (residues 1 to 402)
  AUTHORS   Rice,P.A. and Baker,T.A.
  TITLE     Comparative architecture of transposase and integrase complexes
  JOURNAL   Nat Struct Biol 8 (4), 302-307 (2001)
   PUBMED   11276247
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 11430747
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Enterobacteriaceae"
                     /db_xref="taxon:543"
     Protein         1..402
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /EC_number="3.1.21.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence
                     IEA]"
                     /GO_process="GO:0032196 - transposition [Evidence IEA]"
                     /calculated_mol_wt=45610
     Region          1..392
                     /region_name="InsQ"
                     /note="Transposase [Mobilome: prophages, transposons];
                     COG0675"
                     /db_xref="CDD:440439"
ORIGIN      
        1 mkrlqafkfq lrpggqqece mrrfagacrf vfnralarqn enheagnkyi pygkmaswlv
       61 ewknatetqw lkdspsqplq qslkdleray knffrkraaf prfkkrgqnd afrypqgvkl
      121 dqensriflp klgwmryrns rqvtgvvknv tvsqscgkwy isiqtesevs tpvhpsasmv
      181 gldagvakla tlsdgtvfep vnsfqknqkk larlqrqlsr kvkfsnnwqk qkrkiqrlhs
      241 cianirrdyl hkvttavskn hamiviedlk vsnmsksaag tvsqpgrnvr aksglnrsil
      301 dqgwyemrrq laykqlwrgg qvlavppayt sqrcaycght akenrlsqsk frcqvcgyta
      361 nadvngarni laaghavlac gemvqsgrpl kqeptemiqa ta