MULTISPECIES: bifunctional hydroxymethylpyrimidine

LOCUS       WP_000822268             266 aa            linear   BCT 17-JAN-2025
            kinase/phosphomethylpyrimidine kinase [Escherichia].
ACCESSION   WP_000822268
VERSION     WP_000822268.1
KEYWORDS    RefSeq.
SOURCE      Escherichia
  ORGANISM  Escherichia
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae.
REFERENCE   1  (residues 1 to 266)
  AUTHORS   Mizote,T., Tsuda,M., Smith,D.D., Nakayama,H. and Nakazawa,T.
  TITLE     Cloning and characterization of the thiD/J gene of Escherichia coli
            encoding a thiamin-synthesizing bifunctional enzyme,
            hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
  JOURNAL   Microbiology (Reading, Engl.) 145 (Pt 2), 495-501 (1999)
   PUBMED   10075431
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR00097.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia"
                     /db_xref="taxon:561"
     gene            1..266
                     /gene="thiD"
     Protein         1..266
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /GO_function="GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA]"
                     /GO_function="GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=28487
     Region          1..266
                     /region_name="PRK06427"
                     /note="bifunctional hydroxy-methylpyrimidine kinase/
                     hydroxy-phosphomethylpyrimidine kinase; Reviewed"
                     /db_xref="CDD:180561"
     Site            order(5,12,14..15,17,21,24..25,28,31..36,38,41..45,50..51,
                     53,55..56,59,62,66..67)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:238574"
     Site            order(11,23,44,80,213)
                     /site_type="other"
                     /note="substrate binding site [chemical binding]"
                     /db_xref="CDD:238574"
     Site            order(105,142,176,210,212)
                     /site_type="other"
                     /note="ATP binding site [chemical binding]"
                     /db_xref="CDD:238574"
ORIGIN      
        1 mkrinaltia gtdpsggagi qadlktfsal gaygcsvita lvaqntrgvq svyriepdfv
       61 aaqldsvfsd vridttkigm laetdiveav aerlqryqiq nvvldtvmla ksgdpllsps
      121 avatlrsrll pqvslitpnl peaaalldap harteqemle qgrallamgc gavlmkgghl
      181 ddeqspdwlf tregeqrfta primtknthg tgctlsaala alrprhtnwa dtvqeakswl
      241 ssalaqadtl evghgigpvh hfhaww