MULTISPECIES: bifunctional 4-hydroxy-2-oxoglutarate

LOCUS       WP_000800512             213 aa            linear   BCT 16-DEC-2020
            aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase
            [Enterobacteriaceae].
ACCESSION   WP_000800512
VERSION     WP_000800512.1
KEYWORDS    RefSeq.
SOURCE      Enterobacteriaceae
  ORGANISM  Enterobacteriaceae
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales.
REFERENCE   1  (residues 1 to 213)
  AUTHORS   Walters,M.J., Srikannathasan,V., McEwan,A.R., Naismith,J.H.,
            Fierke,C.A. and Toone,E.J.
  TITLE     Characterization and crystal structure of Escherichia coli KDPGal
            aldolase
  JOURNAL   Bioorg. Med. Chem. 16 (2), 710-720 (2008)
   PUBMED   17981470
REFERENCE   2  (residues 1 to 213)
  AUTHORS   Fullerton,S.W., Griffiths,J.S., Merkel,A.B., Cheriyan,M.,
            Wymer,N.J., Hutchins,M.J., Fierke,C.A., Toone,E.J. and
            Naismith,J.H.
  TITLE     Mechanism of the Class I KDPG aldolase
  JOURNAL   Bioorg. Med. Chem. 14 (9), 3002-3010 (2006)
   PUBMED   16403639
REFERENCE   3  (residues 1 to 213)
  AUTHORS   Wymer,N., Buchanan,L.V., Henderson,D., Mehta,N., Botting,C.H.,
            Pocivavsek,L., Fierke,C.A., Toone,E.J. and Naismith,J.H.
  TITLE     Directed evolution of a new catalytic site in
            2-keto-3-deoxy-6-phosphogluconate aldolase from Escherichia coli
  JOURNAL   Structure 9 (1), 1-9 (2001)
   PUBMED   11342129
REFERENCE   4  (residues 1 to 213)
  AUTHORS   Patil,R.V. and Dekker,E.E.
  TITLE     Cloning, nucleotide sequence, overexpression, and inactivation of
            the Escherichia coli 2-keto-4-hydroxyglutarate aldolase gene
  JOURNAL   J. Bacteriol. 174 (1), 102-107 (1992)
   PUBMED   1339418
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: BlastRule
            Evidence Accession :: NBR011120
            Evidence Source    :: NCBI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..213
                     /organism="Enterobacteriaceae"
                     /db_xref="taxon:543"
     gene            1..213
                     /gene="kdgA"
                     /gene_synonym="eda"
     Protein         1..213
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /calculated_mol_wt=22153
     Region          1..212
                     /region_name="PRK05718"
                     /note="keto-hydroxyglutarate-aldolase/keto-deoxy-
                     phosphogluconate aldolase; Provisional"
                     /db_xref="CDD:235577"
     Site            order(45,49,73,133,135,161)
                     /site_type="active"
                     /db_xref="CDD:188632"
     Site            order(49,73,75,94..97,117..120,122,126,135..136,148,
                     152..153)
                     /site_type="other"
                     /note="intersubunit interface [polypeptide binding]"
                     /db_xref="CDD:188632"
     Site            133
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:188632"
ORIGIN      
        1 mknwktsaes ilttgpvvpv ivvkklehav pmakalvagg vrvlevtlrt ecavdairai
       61 akevpeaivg agtvlnpqql aevteagaqf aispgltepl lkaategtip lipgistvse
      121 lmlgmdyglk efkffpaean ggvkalqaia gpfsqvrfcp tggispanyr dylalksvlc
      181 iggswlvpad aleagdydri tklareaveg akl