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spermidine/putrescine ABC transporter permease PotB [Escherichia


LOCUS       WP_000799385             287 aa            linear   BCT 13-MAR-2021
            coli].
ACCESSION   WP_000799385
VERSION     WP_000799385.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 287)
  AUTHORS   Igarashi,K. and Kashiwagi,K.
  TITLE     Polyamine transport in bacteria and yeast
  JOURNAL   Biochem J 344 Pt 3, 633-642 (1999)
   PUBMED   10585849
REFERENCE   2  (residues 1 to 287)
  AUTHORS   Kashiwagi,K., Endo,H., Kobayashi,H., Takio,K. and Igarashi,K.
  TITLE     Spermidine-preferential uptake system in Escherichia coli. ATP
            hydrolysis by PotA protein and its association with membrane
  JOURNAL   J Biol Chem 270 (43), 25377-25382 (1995)
   PUBMED   7592703
REFERENCE   3  (residues 1 to 287)
  AUTHORS   Kashiwagi,K., Miyamoto,S., Nukui,E., Kobayashi,H. and Igarashi,K.
  TITLE     Functions of potA and potD proteins in spermidine-preferential
            uptake system in Escherichia coli
  JOURNAL   J Biol Chem 268 (26), 19358-19363 (1993)
   PUBMED   8366082
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF007044.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK09497
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..287
                     /gene="potB"
     Protein         1..287
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /EC_number="7.6.2.-"
                     /GO_component="GO:0016020 - membrane [Evidence IEA]"
                     /GO_process="GO:0055085 - transmembrane transport
                     [Evidence IEA]"
                     /calculated_mol_wt=32220
     Region          1..282
                     /region_name="potB"
                     /note="spermidine/putrescine ABC transporter membrane
                     protein; Reviewed; PRK09497"
                     /db_xref="CDD:181910"
     Site            order(81..82,85..86,91,101..104,106..115,118..119,134,
                     159..160,162,164,167..168,170,174..175,178,195,209..210,
                     213,220..223,226..227,238..239,249..250,253..254,256..257,
                     260..261,264)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:119394"
     Site            order(107..120,134,220..225)
                     /site_type="active"
                     /note="conserved gate region [active]"
                     /db_xref="CDD:119394"
     Site            order(134,154,226,236,239,249)
                     /site_type="active"
                     /note="putative PBP binding loops [active]"
                     /db_xref="CDD:119394"
     Site            order(179..191,197..198,202)
                     /site_type="active"
                     /note="ABC-ATPase subunit interface [active]"
                     /db_xref="CDD:119394"
ORIGIN      
        1 mkntskfqnv vivtivgwlv lfvflpnlmi igtsflardd asfvkmvftl dnytrlldpl
       61 yfevllhsln maliatlacl vlgypfawfl aklphkvrpl llfllivpfw tnsliriygl
      121 kiflstkgyl nefllwlgvi dtpirimftp saviiglvyi llpfmvmply ssiekldkpl
      181 leaardlgas klqtfiriii pltmpgiiag cllvmlpamg lfyvsdlmgg aknllignvi
      241 kvqflnirdw pfgaatsitl tivmglmllv ywrasrllnk kateidn