bifunctional UDP-sugar hydrolase/5'-nucleotidase [Escherichia

LOCUS       WP_000771719             550 aa            linear   BCT 02-MAR-2020
            coli].
ACCESSION   WP_000771719
VERSION     WP_000771719.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 550)
  AUTHORS   Krug,U., Patzschke,R., Zebisch,M., Balbach,J. and Strater,N.
  TITLE     Contribution of the two domains of E. coli 5'-nucleotidase to
            substrate specificity and catalysis
  JOURNAL   FEBS Lett. 587 (5), 460-466 (2013)
   PUBMED   23333297
REFERENCE   2  (residues 1 to 550)
  AUTHORS   Knofel,T. and Strater,N.
  TITLE     E. coli 5'-nucleotidase undergoes a hinge-bending domain rotation
            resembling a ball-and-socket motion
  JOURNAL   J. Mol. Biol. 309 (1), 255-266 (2001)
   PUBMED   11491294
REFERENCE   3  (residues 1 to 550)
  AUTHORS   Knofel,T. and Strater,N.
  TITLE     Mechanism of hydrolysis of phosphate esters by the dimetal center
            of 5'-nucleotidase based on crystal structures
  JOURNAL   J. Mol. Biol. 309 (1), 239-254 (2001)
   PUBMED   11491293
REFERENCE   4  (residues 1 to 550)
  AUTHORS   Knofel,T. and Strater,N.
  TITLE     X-ray structure of the Escherichia coli periplasmic 5'-nucleotidase
            containing a dimetal catalytic site
  JOURNAL   Nat. Struct. Biol. 6 (5), 448-453 (1999)
   PUBMED   10331872
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: BlastRule
            Evidence Accession :: NBR011290
            Evidence Source    :: NCBI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..550
                     /gene="ushA"
     Protein         1..550
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /calculated_mol_wt=60600
     Region          1..550
                     /region_name="ushA"
                     /note="bifunctional UDP-sugar hydrolase/5'-nucleotidase
                     periplasmic precursor; Reviewed; PRK09558"
                     /db_xref="CDD:236566"
ORIGIN      
        1 mkllqrgval allttftlas eaalayeqdk tykitvlhtn dhhghfwrne ygeyglaaqk
       61 tlvdgirkev aaeggsvlll sggdintgvp esdlqdaepd frgmnlvgyd amaignhefd
      121 npltvlrqqe kwakfpllsa niyqkstger lfkpwalfkr qdlkiavigl ttddtaklgn
      181 penftdiefr kpadeaklvi qelqqtekpd iiiaathmgh ydngdhgsna pgdvemaral
      241 pagslamivg ghsqdpvcma aenkkqvdyv pgtpckpdqq ngiwivqahe wgkyvgradf
      301 efrngemkmv nyqlipvnlk kkvtwedgks ervlytpeia enqqmislls pfqnkgkaql
      361 evkigetngr legdrdkvrf vqtnmgrlil aaqmdrtgad favmsgggir dsieagdisy
      421 knvlkvqpfg nvvvyadmtg kevidyltav aqmkpdsgay pqfanvsfva kdgklndlki
      481 kgepvdpakt yrmatlnfna tggdgyprld nkpgyvntgf idaevlkayi qksspldvsv
      541 yepkgevswq