spermidine/putrescine ABC transporter substrate-binding protein

LOCUS       WP_000759316             348 aa            linear   BCT 13-MAR-2021
            PotD [Escherichia coli].
ACCESSION   WP_000759316
VERSION     WP_000759316.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 348)
  AUTHORS   Igarashi,K. and Kashiwagi,K.
  TITLE     Polyamine transport in bacteria and yeast
  JOURNAL   Biochem J 344 Pt 3, 633-642 (1999)
   PUBMED   10585849
REFERENCE   2  (residues 1 to 348)
  AUTHORS   Johnston,J.S. and Athwal,D.S.
  TITLE     Recombinant anti-polyamine antibodies: identification of a
            conserved binding site motif
  JOURNAL   Protein Eng 12 (6), 515-521 (1999)
   PUBMED   10388849
REFERENCE   3  (residues 1 to 348)
  AUTHORS   Kashiwagi,K., Endo,H., Kobayashi,H., Takio,K. and Igarashi,K.
  TITLE     Spermidine-preferential uptake system in Escherichia coli. ATP
            hydrolysis by PotA protein and its association with membrane
  JOURNAL   J Biol Chem 270 (43), 25377-25382 (1995)
   PUBMED   7592703
REFERENCE   4  (residues 1 to 348)
  AUTHORS   Kashiwagi,K., Miyamoto,S., Nukui,E., Kobayashi,H. and Igarashi,K.
  TITLE     Functions of potA and potD proteins in spermidine-preferential
            uptake system in Escherichia coli
  JOURNAL   J Biol Chem 268 (26), 19358-19363 (1993)
   PUBMED   8366082
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF007048.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK09501
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..348
                     /gene="potD"
     Protein         1..348
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /EC_number="7.6.2.-"
                     /GO_component="GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /GO_function="GO:0019808 - polyamine binding [Evidence
                     IEA]"
                     /GO_process="GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /calculated_mol_wt=38660
     Region          1..348
                     /region_name="potD"
                     /note="spermidine/putrescine ABC transporter periplasmic
                     substrate-binding protein; Reviewed; PRK09501"
                     /db_xref="CDD:181913"
     Site            order(30,35,40..41,44,55..58,60,216,219..220,235,237)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:270378"
     Site            order(34,229,232,255,257,293)
                     /site_type="other"
                     /note="chemical substrate binding site [chemical binding]"
                     /db_xref="CDD:270378"
ORIGIN      
        1 mkkwsrhlla agalalgmsa ahaddnntly fynwteyvpp glleqftket gikviystye
       61 snetmyaklk tykdgaydlv vpstyyvdkm rkegmiqkid kskltnfsnl dpdmlnkpfd
      121 pnndysipyi wgataigvng davdpksvts wadlwkpeyk gsllltddar evfqmalrkl
      181 gysgnttdpk eieaaynelk klmpnvaafn sdnpanpyme gevnlgmiwn gsafvarqag
      241 tpidvvwpke ggifwmdsla ipanaknkeg alklinfllr pdvakqvaet igyptpnlaa
      301 rkllspevan dktlspdaet ikngewqndv gaassiyeey yqklkagr