MULTISPECIES: tRNA 4-thiouridine(8) synthase ThiI

LOCUS       WP_000668662             482 aa            linear   BCT 28-FEB-2022
            [Enterobacteriaceae].
ACCESSION   WP_000668662
VERSION     WP_000668662.1
KEYWORDS    RefSeq.
SOURCE      Enterobacteriaceae
  ORGANISM  Enterobacteriaceae
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales.
REFERENCE   1  (residues 1 to 482)
  AUTHORS   Martinez-Gomez,N.C., Palmer,L.D., Vivas,E., Roach,P.L. and
            Downs,D.M.
  TITLE     The rhodanese domain of ThiI is both necessary and sufficient for
            synthesis of the thiazole moiety of thiamine in Salmonella enterica
  JOURNAL   J Bacteriol 193 (18), 4582-4587 (2011)
   PUBMED   21724998
REFERENCE   2  (residues 1 to 482)
  AUTHORS   You,D., Xu,T., Yao,F., Zhou,X. and Deng,Z.
  TITLE     Direct evidence that ThiI is an ATP pyrophosphatase for the
            adenylation of uridine in 4-thiouridine biosynthesis
  JOURNAL   Chembiochem 9 (12), 1879-1882 (2008)
   PUBMED   18604845
REFERENCE   3  (residues 1 to 482)
  AUTHORS   Mueller,E.G., Palenchar,P.M. and Buck,C.J.
  TITLE     The role of the cysteine residues of ThiI in the generation of
            4-thiouridine in tRNA
  JOURNAL   J Biol Chem 276 (36), 33588-33595 (2001)
   PUBMED   11443125
REFERENCE   4  (residues 1 to 482)
  AUTHORS   Webb,E., Claas,K. and Downs,D.M.
  TITLE     Characterization of thiI, a new gene involved in thiazole
            biosynthesis in Salmonella typhimurium
  JOURNAL   J Bacteriol 179 (13), 4399-4402 (1997)
   PUBMED   9209060
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR00342.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Enterobacteriaceae"
                     /db_xref="taxon:543"
     gene            1..482
                     /gene="thiI"
     Protein         1..482
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /EC_number="2.8.1.4"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0006400 - tRNA modification [Evidence
                     IEA]"
                     /GO_process="GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=54842
     Region          3..379
                     /region_name="ThiI"
                     /note="Adenylyl- and sulfurtransferase ThiI (thiamine and
                     tRNA 4-thiouridine biosynthesis) [Coenzyme transport and
                     metabolism, Translation, ribosomal structure and
                     biogenesis]; COG0301"
                     /db_xref="CDD:440070"
     Region          382..482
                     /region_name="ThiI_C_thiazole"
                     /note="thiazole biosynthesis domain; TIGR04271"
                     /db_xref="CDD:275094"
     Site            456
                     /site_type="active"
                     /note="active site residue [active]"
                     /db_xref="CDD:238089"
ORIGIN      
        1 mkfiiklfpe itiksqsvrl rfikiltgni rnvlkhydet lavvrhwdni evrakdenqr
       61 lairdaltri pgihhileve dvpftdmhdi fekalvqyrd qlegktfcvr vkrrgkhdfs
      121 sidveryvgg glnqhiesar vkltnpdvtv hleveddrll likgryegig gfpigtqedv
      181 lslisggfds gvssymlmrr gcrvhycffn lggaaheigv rqvahylwnr fgsshrvrfv
      241 ainfepvvge ilekiddgqm gvilkrmmvr aaskvaeryg vqalvtgeal gqvssqtltn
      301 lrlidnvsdt lilrplisyd kehiinlarq igtedfartm peycgvisks ptvkavkski
      361 eaeeekfdfs ildkvveean nvdireiaqq teqevvevet vngfgpndvi ldirsideqe
      421 dkplkvegid vvslpfykls tkfgdldqnk twllwcergv msrlqalylr eqgfnnvkvy
      481 rp