glutamate--cysteine ligase [Escherichia coli].

LOCUS       WP_000611787             518 aa            linear   BCT 25-NOV-2024
ACCESSION   WP_000611787
VERSION     WP_000611787.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 518)
  AUTHORS   Wongsaroj,L., Saninjuk,K., Romsang,A., Duang-Nkern,J.,
            Trinachartvanit,W., Vattanaviboon,P. and Mongkolsuk,S.
  TITLE     Pseudomonas aeruginosa glutathione biosynthesis genes play multiple
            roles in stress protection, bacterial virulence and biofilm
            formation
  JOURNAL   PLoS One 13 (10), e0205815 (2018)
   PUBMED   30325949
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 518)
  AUTHORS   Hibi,T., Nii,H., Nakatsu,T., Kimura,A., Kato,H., Hiratake,J. and
            Oda,J.
  TITLE     Crystal structure of gamma-glutamylcysteine synthetase: insights
            into the mechanism of catalysis by a key enzyme for glutathione
            homeostasis
  JOURNAL   Proc Natl Acad Sci U S A 101 (42), 15052-15057 (2004)
   PUBMED   15477603
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR01434.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..518
                     /gene="gshA"
     Protein         1..518
                     /product="glutamate--cysteine ligase"
                     /EC_number="6.3.2.2"
                     /GO_function="GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA]"
                     /GO_process="GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=58124
     Region          6..517
                     /region_name="glu_cys_ligase"
                     /note="glutamate--cysteine ligase; TIGR01434"
                     /db_xref="CDD:213621"
ORIGIN      
        1 mipdvsqala wlekhpqalk giqrgleret lrvnadgtla ttghpealgs althkwittd
       61 faeallefit pvdgdiehml tfmrdlhryt arnmgdermw plsmpcyiae gqdielaqyg
      121 tsntgrfktl yreglknryg almqtisgvh ynfslpmafw qakcgdisga dakekisagy
      181 frvirnyyrf gwvipylfga spaicssflq gkptslpfek tecgmyylpy atslrlsdlg
      241 ytnksqsnlg itfndlyeyv aglkqaiktp seeyakigid kdgkrlqins nvlqienely
      301 apirpkrvtr sgespsdall rggieyievr sldinpfspi gvdeqqvrfl dlfmvwcala
      361 dapemsssel actrvnwnrv ilegrkpglt lgigcetaqf plpqvgkdlf rdlkrvaqtl
      421 dsinggeayq kvcdelvacf dnpdltfsar ilrsmidtgi ggtgkafaea yrnllreepl
      481 eilreedfva ereaserrqq emeaadtepf avwlekha