tRNA 2-thiouridine(34) synthase MnmA [Shigella flexneri].

LOCUS       WP_000575126             383 aa            linear   BCT 27-DEC-2023
ACCESSION   WP_000575126
VERSION     WP_000575126.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (residues 1 to 383)
  AUTHORS   Numata,T., Ikeuchi,Y., Fukai,S., Suzuki,T. and Nureki,O.
  TITLE     Snapshots of tRNA sulphuration via an adenylated intermediate
  JOURNAL   Nature 442 (7101), 419-424 (2006)
   PUBMED   16871210
REFERENCE   2  (residues 1 to 383)
  AUTHORS   Kambampati,R. and Lauhon,C.T.
  TITLE     MnmA and IscS are required for in vitro 2-thiouridine biosynthesis
            in Escherichia coli
  JOURNAL   Biochemistry 42 (4), 1109-1117 (2003)
   PUBMED   12549933
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: TIGR00420.1
            Evidence Source    :: JCVI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..383
                     /organism="Shigella flexneri"
                     /db_xref="taxon:623"
     gene            1..383
                     /gene="mnmA"
                     /gene_synonym="trmU"
     Protein         1..383
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /EC_number="2.8.1.13"
                     /GO_component="GO:0005737 - cytoplasm [Evidence IEA]"
                     /GO_function="GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA]"
                     /GO_function="GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /GO_process="GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /GO_process="GO:0006396 - RNA processing [Evidence IEA]"
                     /calculated_mol_wt=42506
     Region          21..380
                     /region_name="MnmA"
                     /note="tRNA U34 2-thiouridine synthase MnmA/TrmU, contains
                     the PP-loop ATPase domain [Translation, ribosomal
                     structure and biogenesis]; COG0482"
                     /db_xref="CDD:440250"
ORIGIN      
        1 mieyaalkfn vvsdpmseta kkvivgmsgg vdssvsawll qqqgyqvegl fmknweeddg
       61 eeyctaaadl adaqavcdkl gielhtvnfa aeywdnvfel flaeykagrt pnpdilcnke
      121 ikfkaflefa aedlgadyia tghyvrradv dgksrllrgl dsnkdqsyfl ytlsheqiaq
      181 slfpvgelek pqvrkiaedl glvtakkkds tgicfigerk freflgrylp aqpgkiitvd
      241 gdeigehqgl myhtlgqrkg lgiggtkegt eepwyvvdkd vennilvvaq ghehprlmsv
      301 gliaqqlhwv drepftgtmr ctvktryrqt dipctvkald ddrikvifde pvaavtpgqs
      361 avfyngevcl gggiieqrlp lpv