M20 aminoacylase family protein [Escherichia coli].

LOCUS       WP_000569560             388 aa            linear   BCT 21-MAR-2023
ACCESSION   WP_000569560
VERSION     WP_000569560.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 388)
  AUTHORS   Lindner,H.A., Lunin,V.V., Alary,A., Hecker,R., Cygler,M. and
            Menard,R.
  TITLE     Essential roles of zinc ligation and enzyme dimerization for
            catalysis in the aminoacylase-1/M20 family
  JOURNAL   J Biol Chem 278 (45), 44496-44504 (2003)
   PUBMED   12933810
REFERENCE   2  (residues 1 to 388)
  AUTHORS   Rawlings,N.D. and Barrett,A.J.
  TITLE     Evolutionary families of metallopeptidases
  JOURNAL   Methods Enzymol 248, 183-228 (1995)
   PUBMED   7674922
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 10145370
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..388
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     Protein         1..388
                     /product="M20 aminoacylase family protein"
                     /GO_function="GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /GO_function="GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /calculated_mol_wt=42058
     Region          11..385
                     /region_name="M20_Acy1-like"
                     /note="M20 Peptidase aminoacylase 1 subfamily; cd05666"
                     /db_xref="CDD:349916"
     Site            order(100,102,135,161,361)
                     /site_type="metal-binding"
                     /note="metal binding site [ion binding]"
                     /db_xref="CDD:349916"
     Site            order(184,206..207,211,214,217,234..240,244..245,247,
                     253..255)
                     /site_type="other"
                     /note="putative dimer interface [polypeptide binding]"
                     /db_xref="CDD:349916"
ORIGIN      
        1 miehyirgfe eelreirhqi henpelglqe fktsalvaek lrqwgyeveq glattgivat
       61 lkvgdgeksi glradmdalp iyensgkpwa skhpglmhac ghdghttill gaaryfaetr
      121 rfngtlrlif qpaeemingg eimvkeglfd hfpcdvifgm hnmpglpvgk fyfqpgalma
      181 smdqfhitvr gcgghgaiph kaidpvlvaa hittalqsiv srnvdpleaa vitvgsivag
      241 eaanvipdsa emkisvrsls rdtrqllltr ipalaqaqaa sfgataevth vngtpvlvnd
      301 eemarfawqv acktfgedra efgikplmgs edfsfmleaq pkggfllfgn gdvgegscmv
      361 hnpgydfnda slvpassywg alveawlq