methylenetetrahydrofolate reductase [Vibrio sp. RC586].

LOCUS       WP_000542399             295 aa            linear   BCT 10-DEC-2019
ACCESSION   WP_000542399
VERSION     WP_000542399.1
KEYWORDS    RefSeq.
SOURCE      Vibrio sp. RC586
  ORGANISM  Vibrio sp. RC586
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Vibrionales; Vibrionaceae; Vibrio.
REFERENCE   1  (residues 1 to 295)
  AUTHORS   Trimmer,E.E., Ballou,D.P., Galloway,L.J., Scannell,S.A.,
            Brinker,D.R. and Casas,K.R.
  TITLE     Aspartate 120 of Escherichia coli methylenetetrahydrofolate
            reductase: evidence for major roles in folate binding and catalysis
            and a minor role in flavin reactivity
  JOURNAL   Biochemistry 44 (18), 6809-6822 (2005)
   PUBMED   15865426
REFERENCE   2  (residues 1 to 295)
  AUTHORS   Haller,T., Buckel,T., Retey,J. and Gerlt,J.A.
  TITLE     Discovering new enzymes and metabolic pathways: conversion of
            succinate to propionate by Escherichia coli
  JOURNAL   Biochemistry 39 (16), 4622-4629 (2000)
   PUBMED   10769117
REFERENCE   3  (residues 1 to 295)
  AUTHORS   Guenther,B.D., Sheppard,C.A., Tran,P., Rozen,R., Matthews,R.G. and
            Ludwig,M.L.
  TITLE     The structure and properties of methylenetetrahydrofolate reductase
            from Escherichia coli suggest how folate ameliorates human
            hyperhomocysteinemia
  JOURNAL   Nat. Struct. Biol. 6 (4), 359-365 (1999)
   PUBMED   10201405
REFERENCE   4  (residues 1 to 295)
  AUTHORS   Saint-Girons,I., Duchange,N., Zakin,M.M., Park,I., Margarita,D.,
            Ferrara,P. and Cohen,G.N.
  TITLE     Nucleotide sequence of metF, the E. coli structural gene for 5-10
            methylene tetrahydrofolate reductase and of its control region
  JOURNAL   Nucleic Acids Res. 11 (19), 6723-6732 (1983)
   PUBMED   6356036
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF006950.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK09432
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Vibrio sp. RC586"
                     /db_xref="taxon:675815"
     gene            1..295
                     /gene="metF"
     Protein         1..295
                     /product="methylenetetrahydrofolate reductase"
                     /EC_number="1.5.1.20"
                     /GO_function="GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA]"
                     /GO_process="GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /calculated_mol_wt=32699
     Region          1..295
                     /region_name="metF"
                     /note="methylenetetrahydrofolate reductase; PRK09432"
                     /db_xref="CDD:181852"
     Site            order(59,87,116..118,130..131,149,151,155,157,164,167,
                     170..171,180,182,274)
                     /site_type="other"
                     /note="FAD binding site [chemical binding]"
                     /db_xref="CDD:238299"
ORIGIN      
        1 mgythashid alnqniaels dinvsfeffp psspqmeetl wnsvhrlktl qpkfvsvtyg
       61 ansgerdrth siikaikdqt gliaaphltc idatrdeliq iaddywnngi qnivalrgdi
      121 pagggkpdmy asdlvallks rhdfdisvaa fpevhpeaks aqadllnlkr kvdaganrai
      181 tqfffdvesy lrfrdrcvaa gidveivpgi lpvsnfkqas rfaaqnnvkv pnwmvkqfeg
      241 ldedpvtrql vgasqaidmv rvlcregvkd fhfytlnrae mtyalchtlg vrpqa