alpha-amylase family glycosyl hydrolase [Shigella flexneri].

LOCUS       WP_000491412             750 aa            linear   BCT 01-MAR-2025
ACCESSION   WP_000491412
VERSION     WP_000491412.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (residues 1 to 750)
  AUTHORS   Strobl,S., Maskos,K., Betz,M., Wiegand,G., Huber,R.,
            Gomis-Ruth,F.X. and Glockshuber,R.
  TITLE     Crystal structure of yellow meal worm alpha-amylase at 1.64 A
            resolution
  JOURNAL   J Mol Biol 278 (3), 617-628 (1998)
   PUBMED   9600843
REFERENCE   2  (residues 1 to 750)
  AUTHORS   Larson,S.B., Greenwood,A., Cascio,D., Day,J. and McPherson,A.
  TITLE     Refined molecular structure of pig pancreatic alpha-amylase at 2.1
            A resolution
  JOURNAL   J Mol Biol 235 (5), 1560-1584 (1994)
   PUBMED   8107092
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF012356.6
            Evidence Source    :: EMBL-EBI
            Source Identifier  :: PF00128.30
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..750
                     /organism="Shigella flexneri"
                     /db_xref="taxon:623"
     Protein         1..750
                     /product="alpha-amylase family glycosyl hydrolase"
                     /GO_function="GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /GO_process="GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /calculated_mol_wt=85058
     Region          216..640
                     /region_name="AmyAc_bac1_AmyA"
                     /note="Alpha amylase catalytic domain found in bacterial
                     Alpha-amylases (also called
                     1,4-alpha-D-glucan-4-glucanohydrolase); cd11315"
                     /db_xref="CDD:200454"
     Site            order(268..269,272..273,312,315,390,434,436..437,439..440,
                     481,483,485,550..551,555)
                     /site_type="active"
                     /db_xref="CDD:200454"
     Site            order(311,406,431)
                     /site_type="other"
                     /note="Ca binding site [ion binding]"
                     /db_xref="CDD:200454"
     Site            order(436,481,551)
                     /site_type="active"
                     /note="catalytic site [active]"
                     /db_xref="CDD:200454"
     Region          651..738
                     /region_name="Alpha-amylase_C"
                     /note="Alpha amylase, C-terminal all-beta domain;
                     pfam02806"
                     /db_xref="CDD:426991"
ORIGIN      
        1 mfsikpgpgn lpidnptlls wnitdgdlns kfntleylnc itniinacgv ypqdlkdrei
       61 istfhaekvi ndllkndyki slspdttyre lnkaaqssit apdrigegkt wvyqrdtmve
      121 rgdnsgvhqy gpaehfthii sdkpspkdky vayainipdy elaadvynin vtspsgqqet
      181 fkilinpehl rqtlerkslt avqksqceii tpkkpgeail hafnatyqqi renmsefars
      241 hygyiqippv ttfradgpet peeekgywfh ayqpedlcti hnpmgdlqdf ialvkdakkf
      301 gidiipdytf nfmgiggsgk ndldypsadi rakiskdies gipgywqgqv lipfikdpvt
      361 kerkqihped ihltakdfea skdniskdew knlhalkeks lngmpkttpk sdqvimlqnq
      421 yvremrkygv rglrydaakh skheqiersi tpplknyner lhntnlfnpk yhektvmnym
      481 eylvtcqlne eqmssllyer ddlsaidfsl lmktikafsf ggdlltlask pgstissips
      541 errilininh dfpnngnlfn dflfnhqqde qlamaymaal pfsrplvywd gqvlksttei
      601 knydgstrvg geawlnkgcs tyqqlynefh alyidkagiw safegvfatk nvlafsrgds
      661 vninhsphdg lviinkgnee vegtwpnklq priyknmgsn svniiinntr kiippckvft
      721 lrggslnini prrsalllgk tgeppnylyl