RNA-guided endonuclease InsQ/TnpB family protein [Salmonella

LOCUS       WP_000457373             416 aa            linear   BCT 24-DEC-2024
            enterica].
ACCESSION   WP_000457373
VERSION     WP_000457373.1
KEYWORDS    RefSeq.
SOURCE      Salmonella enterica
  ORGANISM  Salmonella enterica
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Salmonella.
REFERENCE   1  (residues 1 to 416)
  AUTHORS   Karvelis,T., Druteika,G., Bigelyte,G., Budre,K., Zedaveinyte,R.,
            Silanskas,A., Kazlauskas,D., Venclovas,C. and Siksnys,V.
  TITLE     Transposon-associated TnpB is a programmable RNA-guided DNA
            endonuclease
  JOURNAL   Nature 599 (7886), 692-696 (2021)
   PUBMED   34619744
REFERENCE   2  (residues 1 to 416)
  AUTHORS   Siguier,P., Gourbeyre,E. and Chandler,M.
  TITLE     Bacterial insertion sequences: their genomic impact and diversity
  JOURNAL   FEMS Microbiol Rev 38 (5), 865-891 (2014)
   PUBMED   24499397
REFERENCE   3  (residues 1 to 416)
  AUTHORS   Rice,P.A. and Baker,T.A.
  TITLE     Comparative architecture of transposase and integrase complexes
  JOURNAL   Nat Struct Biol 8 (4), 302-307 (2001)
   PUBMED   11276247
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 11430747
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Salmonella enterica"
                     /db_xref="taxon:28901"
     Protein         1..416
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /EC_number="3.1.21.-"
                     /GO_function="GO:0003677 - DNA binding [Evidence IEA]"
                     /GO_function="GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /GO_function="GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /GO_process="GO:0006310 - DNA recombination [Evidence
                     IEA]"
                     /GO_process="GO:0032196 - transposition [Evidence IEA]"
                     /calculated_mol_wt=46710
     Region          41..403
                     /region_name="InsQ"
                     /note="Transposase [Mobilome: prophages, transposons];
                     COG0675"
                     /db_xref="CDD:440439"
ORIGIN      
        1 mfahqnasfp prpegrggke avfrltvfci itfsslicea mkraykyrfy ptteqaella
       61 qtfgcvrfvy nsilrwrtda yyerkekigy lqanarltal kkepefawln dvscvplqqs
      121 lrhqqtafan ffagraaypa fkskrhkqaa eftasafkyr dgklymaknk ipldvrwsrp
      181 lpsvpstvti skdaagryfv sclcefepas lpitssmvgi dvglkdlfvt dtgfrsgnpr
      241 htakyaarla llqrrlskka kgsknrakah lkvarlhaki adcrldalhk atrklindnq
      301 vvcveslkvr nmirnpslsk aiadaswgel vrqlrykgew agrsvvaidq ffpsskrcsc
      361 cgfimkkmpl dvrkwqcpec gtdhdrdvna arnikaagla vlahgepvnp eslkaa