MULTISPECIES: tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase MiaE

LOCUS       WP_000218459             270 aa            linear   BCT 17-FEB-2022
            [Salmonella].
ACCESSION   WP_000218459
VERSION     WP_000218459.1
KEYWORDS    RefSeq.
SOURCE      Salmonella
  ORGANISM  Salmonella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae.
REFERENCE   1  (residues 1 to 270)
  AUTHORS   Persson,B.C. and Bjork,G.R.
  TITLE     Isolation of the gene (miaE) encoding the hydroxylase involved in
            the synthesis of 2-methylthio-cis-ribozeatin in tRNA of Salmonella
            typhimurium and characterization of mutants
  JOURNAL   J Bacteriol 175 (24), 7776-7785 (1993)
   PUBMED   8253666
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: BlastRule
            Evidence Accession :: NBR000825
            Evidence Source    :: NCBI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Salmonella"
                     /db_xref="taxon:590"
     gene            1..270
                     /gene="miaE"
     Protein         1..270
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /calculated_mol_wt=31009
     Region          21..264
                     /region_name="MiaE"
                     /note="tRNA-(MS[2]IO[6]A)-hydroxylase (MiaE); pfam06175"
                     /db_xref="CDD:114868"
     Site            order(59,137,140,168,182,185,190,219,222,226)
                     /site_type="active"
                     /db_xref="CDD:153119"
     Site            order(59,137,140,190,219,222)
                     /site_type="other"
                     /note="dinuclear metal binding site [ion binding]"
                     /db_xref="CDD:153119"
     Site            order(60,67,70,128,131..132,135,138,141..142)
                     /site_type="other"
                     /note="dimerization interface [polypeptide binding]"
                     /db_xref="CDD:153119"
ORIGIN      
        1 mtvrqrllsy ffnrlrmnyp qilspvlnfl hcptpqawiv qardpqnlpl lltdhlicel
       61 kaaqtalllv rkyvadksga dallswlqpy eafafrqgpe pdfvalhkqi sksampqtdd
      121 pwgrqlidrm vllikeelhh fwqvrevmqa rnipyvkita sryakgmlka vrthepltli
      181 dklicgayie arscerfaal apwldedlqt fylsllrsea rhyqdylala qqisaedisa
      241 rvryfgevea dlilspdref rfhsgvpaag