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MULTISPECIES: LacI family DNA-binding transcriptional regulator


LOCUS       WP_000201080             335 aa            linear   BCT 29-MAR-2023
            [Salmonella].
ACCESSION   WP_000201080
VERSION     WP_000201080.1
KEYWORDS    RefSeq.
SOURCE      Salmonella
  ORGANISM  Salmonella
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae.
REFERENCE   1  (residues 1 to 335)
  AUTHORS   Bondos,S.E., Swint-Kruse,L. and Matthews,K.S.
  TITLE     Flexibility and Disorder in Gene Regulation: LacI/GalR and Hox
            Proteins
  JOURNAL   J Biol Chem 290 (41), 24669-24677 (2015)
   PUBMED   26342073
REFERENCE   2  (residues 1 to 335)
  AUTHORS   Swint-Kruse,L. and Matthews,K.S.
  TITLE     Allostery in the LacI/GalR family: variations on a theme
  JOURNAL   Curr Opin Microbiol 12 (2), 129-137 (2009)
   PUBMED   19269243
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: Conserved Domain (CDD)
            Evidence Accession :: Domain architecture ID 11265705
            Evidence Source    :: NCBI SPARCLE
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Salmonella"
                     /db_xref="taxon:590"
     Protein         1..335
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /GO_function="GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA]"
                     /GO_function="GO:0003700 - DNA-binding transcription
                     factor activity [Evidence IEA]"
                     /GO_function="GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /GO_process="GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /GO_process="GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /GO_process="GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /calculated_mol_wt=37119
     Region          2..71
                     /region_name="HTH_LACI"
                     /note="helix_turn _helix lactose operon repressor;
                     smart00354"
                     /db_xref="CDD:197675"
     Site            order(5,13..17,19..20,23,28..29,32,45,48,51..52,54..55)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:143331"
     Site            45..54
                     /site_type="active"
                     /note="domain linker motif [active]"
                     /db_xref="CDD:143331"
     Region          61..327
                     /region_name="PBP1_GalS-like"
                     /note="ligand binding domain of DNA transcription
                     iso-repressor GalS, which is one of two regulatory
                     proteins involved in galactose transport and metabolism;
                     cd06270"
                     /db_xref="CDD:380494"
     Site            order(61,68,74,79,82,91..94,96,110,114,221..222,254,
                     280..281)
                     /site_type="other"
                     /note="dimer interface (closed form) [polypeptide
                     binding]"
                     /db_xref="CDD:380494"
     Site            order(73,122,194,219,245,273)
                     /site_type="other"
                     /note="ligand binding site [chemical binding]"
                     /db_xref="CDD:380494"
ORIGIN      
        1 matikdvasm agvstatvsr vinqtawvep vtrervekam rdlnyrrnaa aialakrsgd
       61 mlglltgnla dpffarlarg vedvsrkqqy rlmvcsgghd eemekagldf lvnqgceaiv
      121 vhasrlpdke llryaahfpa lvvvnryiag manrciwlen rsaareatry llanghrria
      181 cvtsdlpiid rqerldgyre aleeygispd prwvisvpfn eeggeraahq linsglplta
      241 avtfndvmaa gimrilhqrg vqlpqqlsiv gfddvvlary lypalttmhy pveqmarcaa
      301 qlaiqlyqgi tpppssnhfn aelvirdsva pyfsr