nitroreductase NfsA [Escherichia coli].

FASTA View on NCBI
LOCUS       WP_000189187             240 aa            linear   BCT 10-JUL-2019
ACCESSION   WP_000189187
VERSION     WP_000189187.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 240)
  AUTHORS   Shanmugam,D., Esak,S.B. and Narayanaswamy,A.
  TITLE     Molecular Characterisation of nfsA Gene in Nitrofurantoin Resistant
            Uropathogens
  JOURNAL   J Clin Diagn Res 10 (6), DC05-DC09 (2016)
   PUBMED   27504284
REFERENCE   2  (residues 1 to 240)
  AUTHORS   Nokhbeh,M.R., Boroumandi,S., Pokorny,N., Koziarz,P., Paterson,E.S.
            and Lambert,I.B.
  TITLE     Identification and characterization of SnrA, an inducible
            oxygen-insensitive nitroreductase in Salmonella enterica serovar
            Typhimurium TA1535
  JOURNAL   Mutat. Res. 508 (1-2), 59-70 (2002)
   PUBMED   12379462
REFERENCE   3  (residues 1 to 240)
  AUTHORS   Zenno,S., Koike,H., Kumar,A.N., Jayaraman,R., Tanokura,M. and
            Saigo,K.
  TITLE     Biochemical characterization of NfsA, the Escherichia coli major
            nitroreductase exhibiting a high amino acid sequence homology to
            Frp, a Vibrio harveyi flavin oxidoreductase
  JOURNAL   J. Bacteriol. 178 (15), 4508-4514 (1996)
   PUBMED   8755878
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: BlastRule
            Evidence Accession :: NBR006554
            Evidence Source    :: NCBI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..240
                     /gene="nfsA"
     Protein         1..240
                     /product="nitroreductase NfsA"
                     /EC_number="1.5.1.38"
                     /calculated_mol_wt=26720
     Region          1..240
                     /region_name="PRK10765"
                     /note="oxygen-insensitive NADPH nitroreductase"
                     /db_xref="CDD:182710"
     Site            order(4..5,7..9,11,29,31..33,35..38,41..44,46..53,56,82,
                     101..103,105..106,109..110,113..114,117,121..122,128,133,
                     143..144,146,153,167..170,172,174..177,192,212..213,216,
                     220..225)
                     /site_type="other"
                     /note="homodimer interface [polypeptide binding]"
                     /db_xref="CDD:380322"
     Site            order(11..13,15,38..40,42,67,69,106..107,110,127..131,167,
                     169)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:380322"
     Site            order(15,42,133..134,165,167,199..200,203)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:380322"
ORIGIN      
        1 mtptieltcg hrsirhftde piseaqreai insaratsss sflqcssiir itdkalreel
       61 vtltggqkhv aqaaefwvfc adfnrhlqic pdaqlglaeq lllgvvdtam maqnaltaae
      121 slglggvyig glrnnieavt kllklpqhvl plfglclgwp adnpdlkprl pssilvhens
      181 yqpldkdala qydeqlaeyy ltrgsnnrrd twsdhirrti ikesrpfild ylhkqgwatr