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LOCUS WP_000148051 165 aa linear BCT 19-FEB-2025 ACCESSION WP_000148051 VERSION WP_000148051.1 KEYWORDS RefSeq. SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (residues 1 to 165) AUTHORS Benjdia,A., Guillot,A., Lefranc,B., Vaudry,H., Leprince,J. and Berteau,O. TITLE Thioether bond formation by SPASM domain radical SAM enzymes: Calpha H-atom abstraction in subtilosin A biosynthesis JOURNAL Chem Commun (Camb) 52 (37), 6249-6252 (2016) PUBMED 27087315 REFERENCE 2 (residues 1 to 165) AUTHORS Grell,T.A., Goldman,P.J. and Drennan,C.L. TITLE SPASM and twitch domains in S-adenosylmethionine (SAM) radical enzymes JOURNAL J Biol Chem 290 (7), 3964-3971 (2015) PUBMED 25477505 REFERENCE 3 (residues 1 to 165) AUTHORS Frey,P.A., Hegeman,A.D. and Ruzicka,F.J. TITLE The Radical SAM Superfamily JOURNAL Crit Rev Biochem Mol Biol 43 (1), 63-88 (2008) PUBMED 18307109 REFERENCE 4 (residues 1 to 165) AUTHORS Booker,S.J., Cicchillo,R.M. and Grove,T.L. TITLE Self-sacrifice in radical S-adenosylmethionine proteins JOURNAL Curr Opin Chem Biol 11 (5), 543-552 (2007) PUBMED 17936058 REFERENCE 5 (residues 1 to 165) AUTHORS Wang,S.C. and Frey,P.A. TITLE S-adenosylmethionine as an oxidant: the radical SAM superfamily JOURNAL Trends Biochem Sci 32 (3), 101-110 (2007) PUBMED 17291766 REMARK Erratum:[Trends Biochem Sci. 2007 May;32(5):209] REFERENCE 6 (residues 1 to 165) AUTHORS Layer,G., Heinz,D.W., Jahn,D. and Schubert,W.D. TITLE Structure and function of radical SAM enzymes JOURNAL Curr Opin Chem Biol 8 (5), 468-476 (2004) PUBMED 15450488 REFERENCE 7 (residues 1 to 165) AUTHORS Sofia,H.J., Chen,G., Hetzler,B.G., Reyes-Spindola,J.F. and Miller,N.E. TITLE Radical SAM, a novel protein superfamily linking unresolved steps in familiar biosynthetic pathways with radical mechanisms: functional characterization using new analysis and information visualization methods JOURNAL Nucleic Acids Res 29 (5), 1097-1106 (2001) PUBMED 11222759 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: Conserved Domain (CDD) Evidence Accession :: Domain architecture ID 11428255 Evidence Source :: NCBI SPARCLE ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..165 /organism="Escherichia coli" /db_xref="taxon:562" Protein 1..165 /product="radical SAM protein" /GO_function="GO:0003824 - catalytic activity [Evidence IEA]" /GO_function="GO:0051539 - 4 iron, 4 sulfur cluster binding [Evidence IEA]" /GO_function="GO:1904047 - S-adenosyl-L-methionine binding [Evidence IEA]" /calculated_mol_wt=19002 Region <17..165 /region_name="PRK13745" /note="anaerobic sulfatase-maturation protein" /db_xref="CDD:237489" ORIGIN 1 mtgsqvidae edrhklvvey kdalqpadfy hnfkqrgirs vqliphlefd drgdmtaasv 61 taelwgkfli alfecwvrad isrisielfd atlqkwcgse npqprrgcqa cdwhllcpha 121 reetpdsvlc agyqafysys aphmrvmrdl ikqhrspmel mtmlr