Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]

glycerol-3-phosphate 1-O-acyltransferase PlsB [Shigella flexneri].


LOCUS       WP_000141711             827 aa            linear   BCT 10-DEC-2019
ACCESSION   WP_000141711
VERSION     WP_000141711.1
KEYWORDS    RefSeq.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Shigella.
REFERENCE   1  (residues 1 to 827)
  AUTHORS   Spoering,A.L., Vulic,M. and Lewis,K.
  TITLE     GlpD and PlsB participate in persister cell formation in
            Escherichia coli
  JOURNAL   J. Bacteriol. 188 (14), 5136-5144 (2006)
   PUBMED   16816185
REFERENCE   2  (residues 1 to 827)
  AUTHORS   Larson,T.J., Ludtke,D.N. and Bell,R.M.
  TITLE     sn-Glycerol-3-phosphate auxotrophy of plsB strains of Escherichia
            coli: evidence that a second mutation, plsX, is required
  JOURNAL   J. Bacteriol. 160 (2), 711-717 (1984)
   PUBMED   6094487
REFERENCE   3  (residues 1 to 827)
  AUTHORS   Green,P.R., Merrill,A.H. Jr. and Bell,R.M.
  TITLE     Membrane phospholipid synthesis in Escherichia coli. Purification,
            reconstitution, and characterization of sn-glycerol-3-phosphate
            acyltransferase
  JOURNAL   J. Biol. Chem. 256 (21), 11151-11159 (1981)
   PUBMED   7026564
REFERENCE   4  (residues 1 to 827)
  AUTHORS   Larson,T.J., Lightner,V.A., Green,P.R., Modrich,P. and Bell,R.M.
  TITLE     Membrane phospholipid synthesis in Escherichia coli. Identification
            of the sn-glycerol-3-phosphate acyltransferase polypeptide as the
            plsB gene product
  JOURNAL   J. Biol. Chem. 255 (19), 9421-9426 (1980)
   PUBMED   6997313
REFERENCE   5  (residues 1 to 827)
  AUTHORS   Lightner,V.A., Larson,T.J., Tailleur,P., Kantor,G.D., Raetz,C.R.,
            Bell,R.M. and Modrich,P.
  TITLE     Membrane phospholipid synthesis in Escherichia coli. Cloning of a
            structural gene (plsB) of the sn-glycerol-3-phosphate
            acyl/transferase
  JOURNAL   J. Biol. Chem. 255 (19), 9413-9420 (1980)
   PUBMED   6251087
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF003441.0
            Evidence Source    :: NCBI Protein Cluster (PRK)
            Source Identifier  :: PRK04974
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Shigella flexneri"
                     /db_xref="taxon:623"
     gene            1..827
                     /gene="plsB"
     Protein         1..827
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /EC_number="2.3.1.15"
                     /GO_function="GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA]"
                     /GO_process="GO:0008654 - phospholipid biosynthetic
                     process [Evidence IEA]"
                     /calculated_mol_wt=93600
     Region          21..826
                     /region_name="PRK04974"
                     /note="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /db_xref="CDD:235325"
     Site            order(326,329,331,352..355,406..408)
                     /site_type="active"
                     /note="putative acyl-acceptor binding pocket [active]"
                     /db_xref="CDD:153255"
ORIGIN      
        1 mtfcypcraf alltrgftsf msgwpriyyk llnlplsilv ksksipadpa pelgldtsrp
       61 imyvlpynsk adlltlraqc lahdlpdple pleidgtllp ryvfihggpr vftyytpkee
      121 siklfhdyld lhrsnpnldv qmvpvsvmfg rapgrekgev npplrmlngv qkffavlwlg
      181 rdsfvrfsps vslrrmadeh gtdktiaqkl arvarmhfar qrlaavgprl parqdlfnkl
      241 lasraiakav edearskkis hekaqqnaia lmeeiaanfs yemirltdri lgftwnrfyq
      301 ginvhnaerv rqlahdghel vyvpchrshm dylllsyvly hqglvpphia aginlnfwpa
      361 gpifrrlgaf firrtfkgnk lystvfreyl gelfsrgysv eyfveggrsr tgrlldpktg
      421 tlsmtiqaml rggtrpitli piyigyehvm evgtyakelr gatkekeslp qmlrglsklr
      481 nlgqgyvnfg epmplmtyln qhvpdwresi dpieavrpaw ltptvnniaa dlmvrinnag
      541 aanamnlcct allasrqrsl treqlteqln cyldlmrnvp ystdstvpsa saselidhal
      601 qmnkfevekd tigdiiilpr eqavlmtyyr nniahmlvlp slmaaivtqh rhisrdvlme
      661 hvnvlypmlk aelflrwdrd elpdvidala nemqrqglit lqddelhinp ahsrtlqlla
      721 agaretlqry aitfwllsan psinrgtlek esrtvaqrls vlhginapef fdkavfsslv
      781 ltlrdegyis dsgdaepaet mkvyqllael itsdvrltie satqgeg