NADPH-dependent 2,4-dienoyl-CoA reductase [Escherichia coli].

LOCUS       WP_000121479             672 aa            linear   BCT 10-FEB-2020
ACCESSION   WP_000121479
VERSION     WP_000121479.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 672)
  AUTHORS   Tu,X., Hubbard,P.A., Kim,J.J. and Schulz,H.
  TITLE     Two distinct proton donors at the active site of Escherichia coli
            2,4-dienoyl-CoA reductase are responsible for the formation of
            different products
  JOURNAL   Biochemistry 47 (4), 1167-1175 (2008)
   PUBMED   18171025
REFERENCE   2  (residues 1 to 672)
  AUTHORS   Hubbard,P.A., Liang,X., Schulz,H. and Kim,J.J.
  TITLE     The crystal structure and reaction mechanism of Escherichia coli
            2,4-dienoyl-CoA reductase
  JOURNAL   J. Biol. Chem. 278 (39), 37553-37560 (2003)
   PUBMED   12840019
REFERENCE   3  (residues 1 to 672)
  AUTHORS   Liang,X., Thorpe,C. and Schulz,H.
  TITLE     2,4-Dienoyl-CoA reductase from Escherichia coli is a novel
            iron-sulfur flavoprotein that functions in fatty acid
            beta-oxidation
  JOURNAL   Arch. Biochem. Biophys. 380 (2), 373-379 (2000)
   PUBMED   10933894
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: BlastRule
            Evidence Accession :: NBR011095
            Evidence Source    :: NCBI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..672
                     /gene="fadH"
     Protein         1..672
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /EC_number="1.3.1.34"
                     /calculated_mol_wt=72448
     Region          6..358
                     /region_name="DCR_FMN"
                     /note="2,4-dienoyl-CoA reductase (DCR) FMN-binding domain.
                     DCR in E. coli is an iron-sulfur flavoenzyme which
                     contains FMN, FAD, and a 4Fe-4S cluster. It is also a
                     monomer, unlike that of its eukaryotic counterparts which
                     form homotetramers and lack the...; cd02930"
                     /db_xref="CDD:239240"
     Site            order(25,27,59,101,165,167,176,215,253,256,311..312,335,
                     338,342,354)
                     /site_type="active"
                     /db_xref="CDD:239240"
     Site            order(25,27,59,101,215,311..312)
                     /site_type="other"
                     /note="FMN binding site [chemical binding]"
                     /db_xref="CDD:239240"
     Site            order(165,167,176,253,256)
                     /site_type="other"
                     /note="2,4-decadienoyl-CoA binding site"
                     /db_xref="CDD:239240"
     Site            167
                     /site_type="active"
                     /note="catalytic residue [active]"
                     /db_xref="CDD:239240"
     Site            order(335,338,342,354)
                     /site_type="other"
                     /note="4Fe-4S cluster binding site [ion binding]"
                     /db_xref="CDD:239240"
     Region          <375..>512
                     /region_name="GltD"
                     /note="NADPH-dependent glutamate synthase beta chain or
                     related oxidoreductase [Amino acid transport and
                     metabolism, General function prediction only]; COG0493"
                     /db_xref="CDD:440259"
     Region          399..>652
                     /region_name="FadH2"
                     /note="NADPH-dependent 2,4-dienoyl-CoA reductase, sulfur
                     reductase, or a related oxidoreductase [Lipid transport
                     and metabolism]; COG0446"
                     /db_xref="CDD:440215"
ORIGIN      
        1 msypslfapl dlgfttlknr vlmgsmhtgl eeypdgaerl aafyaerarh gvalivsggi
       61 apdltgvgme ggamlndasq iphhrtitea vhqeggkial qilhtgrysy qphlvapsal
      121 qapinrfvph eltheeilql iddfarcaql areagydgve vmgsegylin efltlrtnqr
      181 sdqwggdyhn rmrfavevvs avrervgndf iiiyrlsmld lvegggtfae tvelaqaiea
      241 agatiintgi gwhearipti atpvprgafs wvtrklkghv slplvttnri ndpqvaddil
      301 srgdadmvsm arpfladael lskaqsgrad eintcigcnq acldqifvgk vtsclvnpra
      361 chetkmpilp amqkknlavv gagpaglafa inaaarghqv tlfdahseig gqfniakqip
      421 gkeefyetlr yyrrmievtg vtlklnhtvt adqlqafdet ilasgivprt ppidgidhpk
      481 vlsyldvlrd kapvgnkvai igcggigfdt amylsqpges tsqniagfcn ewgidsslqq
      541 agglspqgmq iprsprqivm lqrkaskpgq glgkttgwih rttllsrgvk mipgvsyqki
      601 dddglhvvin getqvlavdn vvicagqepn ralaqplids gktvhliggc dvameldarr
      661 aiaqgtrlal ei