Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]
LOCUS WP_000110082 584 aa linear BCT 29-AUG-2024 ACCESSION WP_000110082 VERSION WP_000110082.1 KEYWORDS RefSeq. SOURCE Escherichia coli ORGANISM Escherichia coli Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (residues 1 to 584) AUTHORS Janscak,P. and Bickle,T.A. TITLE The DNA recognition subunit of the type IB restriction-modification enzyme EcoAI tolerates circular permutions of its polypeptide chain JOURNAL J Mol Biol 284 (4), 937-948 (1998) PUBMED 9837717 REFERENCE 2 (residues 1 to 584) AUTHORS Bickle,T.A. and Kruger,D.H. TITLE Biology of DNA restriction JOURNAL Microbiol Rev 57 (2), 434-450 (1993) PUBMED 8336674 COMMENT REFSEQ: This record represents a single, non-redundant, protein sequence which may be annotated on many different RefSeq genomes from the same, or different, species. ##Evidence-For-Name-Assignment-START## Evidence Category :: HMM Evidence Accession :: NF013579.5 Evidence Source :: EMBL-EBI Source Identifier :: PF01420.24 ##Evidence-For-Name-Assignment-END## COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..584 /organism="Escherichia coli" /db_xref="taxon:562" Protein 1..584 /product="restriction endonuclease subunit S" /EC_number="3.1.21.-" /GO_function="GO:0003677 - DNA binding [Evidence IEA]" /GO_process="GO:0006304 - DNA modification [Evidence IEA]" /calculated_mol_wt=65973 Region 100..577 /region_name="PRK09737" /note="type I restriction-modification system specificity subunit" /db_xref="CDD:236619" Region <244..287 /region_name="RMtype1_S_TRD-CR_like" /note="Type I restriction-modification system specificity (S) subunit Target Recognition Domain-ConseRved domain (TRD-CR) and similar domains; cl38903" /db_xref="CDD:365779" Region 387..578 /region_name="RMtype1_S_Eco933I-TRD2-CR2_like" /note="Type I restriction-modification system specificity (S) subunit TRD-CR, similar to Escherichia coli O157:H7 EDL933 S subunit (S.Eco933I), Escherichia coli O104:H4 2009EL-2071 S subunit (S.Eco2071ORF3585P) TRD2-CR2, and Streptomyces species SirexAA-E S subu; cd17253" /db_xref="CDD:341142" Site order(399..402,421..422,426,446..448,459..461,482..483) /site_type="other" /note="putative DNA binding site [nucleotide binding]" /db_xref="CDD:341142" Site order(498,501..502,504..505,534,536,542,546,549..550, 553..554,556..557,564,568,571,573..574) /site_type="other" /note="putative TRD-CR/TRD-CR interface [polypeptide binding]" /db_xref="CDD:341142" Site order(509..511,513..519,528) /site_type="other" /note="putative HsdM interface [polypeptide binding]" /db_xref="CDD:341142" ORIGIN 1 msveklivdh metwtsalqt rstagrgssg kidlygikkl relilelavr gklvpqdpnd 61 epasellkri aaekaelvkq gkikkqkplp eiseeekpfe lpegwewvri seighdwgqk 121 tpdkdftyid vgsinkeygi ieelsilsak dapsrarkiv qqgtiiystv rpyllniaii 181 eneilpepia stafaiihpy tamdanfiyy ylrspvfvcy vencqtgvay paindkqffs 241 gitpvppsle qvriankike lmslcdqleq qsltsldahq qlvetllgtl tdsqtaeela 301 enwariseyf dtlftteasv dalkqtilql avmgklvpqd pndepasell kriaqekaql 361 vkegkikkqk plppisdeek pfelpegwew clfediidiq sgitkgrnls nrtlvkvpyl 421 rvanvqrgyl dlteikqiei pieekekyqv vkgdlliteg gdwdtvgrtt vwchdwyian 481 qnhvfkgrni gqdvdpywle tymnspfsrq yfanaskqtt nlasinktql rgcpvaipps 541 seakkimskl hifyklceel knhiqsaqqt qlhladaltd aavn