MULTISPECIES: GDP-L-fucose synthase [Enterobacteriaceae].

LOCUS       WP_000089911             321 aa            linear   BCT 14-JAN-2025
ACCESSION   WP_000089911
VERSION     WP_000089911.1
KEYWORDS    RefSeq.
SOURCE      Enterobacteriaceae
  ORGANISM  Enterobacteriaceae
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales.
REFERENCE   1  (residues 1 to 321)
  AUTHORS   Rosano,C., Bisso,A., Izzo,G., Tonetti,M., Sturla,L., De Flora,A.
            and Bolognesi,M.
  TITLE     Probing the catalytic mechanism of GDP-4-keto-6-deoxy-d-mannose
            Epimerase/Reductase by kinetic and crystallographic
            characterization of site-specific mutants
  JOURNAL   J Mol Biol 303 (1), 77-91 (2000)
   PUBMED   11021971
REFERENCE   2  (residues 1 to 321)
  AUTHORS   Menon,S., Stahl,M., Kumar,R., Xu,G.Y. and Sullivan,F.
  TITLE     Stereochemical course and steady state mechanism of the reaction
            catalyzed by the GDP-fucose synthetase from Escherichia coli
  JOURNAL   J Biol Chem 274 (38), 26743-26750 (1999)
   PUBMED   10480878
REFERENCE   3  (residues 1 to 321)
  AUTHORS   Andrianopoulos,K., Wang,L. and Reeves,P.R.
  TITLE     Identification of the fucose synthetase gene in the colanic acid
            gene cluster of Escherichia coli K-12
  JOURNAL   J Bacteriol 180 (4), 998-1001 (1998)
   PUBMED   9473059
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: HMM
            Evidence Accession :: NF047922.1
            Evidence Source    :: NCBIFAM
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterobacteriaceae"
                     /db_xref="taxon:543"
     gene            1..321
                     /gene="fcl"
                     /gene_synonym="wcaG; yefB"
     Protein         1..321
                     /product="GDP-L-fucose synthase"
                     /GO_function="GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /GO_function="GO:0050577 - GDP-L-fucose synthase activity
                     [Evidence IEA]"
                     /GO_process="GO:0042351 - 'de novo' GDP-L-fucose
                     biosynthetic process [Evidence IEA]"
                     /calculated_mol_wt=36052
     Region          5..313
                     /region_name="GDP_FS_SDR_e"
                     /note="GDP-fucose synthetase, extended (e) SDRs; cd05239"
                     /db_xref="CDD:187550"
     Site            order(10,12..15,36,39..42,62..64,66,86,105..107,136,140,
                     163..164,166)
                     /site_type="other"
                     /note="NADP binding site [chemical binding]"
                     /db_xref="CDD:187550"
     Site            order(83,107,136,140)
                     /site_type="active"
                     /db_xref="CDD:187550"
     Site            order(107,136,165,184,201,209)
                     /site_type="other"
                     /note="putative substrate binding site [chemical binding]"
                     /db_xref="CDD:187550"
ORIGIN      
        1 msrqrifiag hrgmvgsair rqleqrgdve lvlrtrdeln lldsravhdf faseridqvy
       61 laaakvggiv anntypadfi yqnmmiesni ihaahqndvn kllflgssci ypklakqpma
      121 esellqgtle ptnepyaiak iagiklcesy nrqygrdyrs vmptnlygph dnfhpsnshv
      181 ipallrrfhe ataqnapdvv vwgsgtpmre flhvddmaaa sihvmelahe vwlentqpml
      241 shinvgtgvd ctirelaqti akvvgykgrv vfdaskpdgt prklldvtrl hqlgwyheis
      301 leaglastyq wflenqdrfr g