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nitrate transporter NarK [Escherichia coli].


LOCUS       WP_000019819             463 aa            linear   BCT 20-FEB-2020
ACCESSION   WP_000019819
VERSION     WP_000019819.1
KEYWORDS    RefSeq.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 463)
  AUTHORS   Zheng,H., Wisedchaisri,G. and Gonen,T.
  TITLE     Crystal structure of a nitrate/nitrite exchanger
  JOURNAL   Nature 497 (7451), 647-651 (2013)
   PUBMED   23665960
  REMARK    Erratum:[Nature. 2014 Mar 13;507(7491):262]
REFERENCE   2  (residues 1 to 463)
  AUTHORS   Giffin,M.M., Raab,R.W., Morganstern,M. and Sohaskey,C.D.
  TITLE     Mutational analysis of the respiratory nitrate transporter NarK2 of
            Mycobacterium tuberculosis
  JOURNAL   PLoS ONE 7 (9), e45459 (2012)
   PUBMED   23029022
REFERENCE   3  (residues 1 to 463)
  AUTHORS   Sohaskey,C.D. and Modesti,L.
  TITLE     Differences in nitrate reduction between Mycobacterium tuberculosis
            and Mycobacterium bovis are due to differential expression of both
            narGHJI and narK2
  JOURNAL   FEMS Microbiol. Lett. 290 (2), 129-134 (2009)
   PUBMED   19076631
REFERENCE   4  (residues 1 to 463)
  AUTHORS   Sohaskey,C.D.
  TITLE     Regulation of nitrate reductase activity in Mycobacterium
            tuberculosis by oxygen and nitric oxide
  JOURNAL   Microbiology (Reading, Engl.) 151 (Pt 11), 3803-3810 (2005)
   PUBMED   16272401
REFERENCE   5  (residues 1 to 463)
  AUTHORS   Jia,W. and Cole,J.A.
  TITLE     Nitrate and nitrite transport in Escherichia coli
  JOURNAL   Biochem. Soc. Trans. 33 (Pt 1), 159-161 (2005)
   PUBMED   15667293
REFERENCE   6  (residues 1 to 463)
  AUTHORS   Clegg,S., Yu,F., Griffiths,L. and Cole,J.A.
  TITLE     The roles of the polytopic membrane proteins NarK, NarU and NirC in
            Escherichia coli K-12: two nitrate and three nitrite transporters
  JOURNAL   Mol. Microbiol. 44 (1), 143-155 (2002)
   PUBMED   11967075
COMMENT     REFSEQ: This record represents a single, non-redundant, protein
            sequence which may be annotated on many different RefSeq genomes
            from the same, or different, species.
            
            ##Evidence-For-Name-Assignment-START##
            Evidence Category  :: BlastRule
            Evidence Accession :: NBR007265
            Evidence Source    :: NCBI
            ##Evidence-For-Name-Assignment-END##
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /db_xref="taxon:562"
     gene            1..463
                     /gene="narK"
     Protein         1..463
                     /product="nitrate transporter NarK"
                     /calculated_mol_wt=49520
     Region          8..463
                     /region_name="MFS"
                     /note="Major Facilitator Superfamily; cl28910"
                     /db_xref="CDD:475125"
     Site            order(48..49,52..53,56,89,144..145,147..149,152,171,
                     174..175,178,263,266..267,270..272,275,301,305,361..362,
                     366,370,407,410..411,414..415,418)
                     /site_type="other"
                     /note="putative chemical substrate binding pocket
                     [chemical binding]"
                     /db_xref="CDD:340899"
ORIGIN      
        1 mshssapera tgavitdwrp edpafwqqrg qriasrnlwi svpclllafc vwmlfsavav
       61 nlpkvgfnft tdqlfmltal psvsgallrv pysfmvpifg grrwtafstg iliipcvwlg
      121 favqdtstpy svfiiisllc gfaganfass manisfffpk qkqggalgln gglgnmgvsv
      181 mqlvaplvvs lsifavfgsq gvkqpdgtel ylanaswiwg pflaiftiaa wfgmndlats
      241 kasikeqlpv lkrghlwims llylatfgsf igfsagfaml sktqfpdvqi lqyaffgpfi
      301 galarsagga lsdrlggtrv tlvnfilmai fsgllfltlp tdgqggsfma ffavflalfl
      361 taglgsgstf qmisvifrkl tmdrvkaegg sderamreaa tdtaaalgfi saigaiggff
      421 ipkafgssla ltgspvgamk vflifyiacv vitwavygrh skk