Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]

apo-serine activating enzyme [Escherichia coli str. K-12 substr.


LOCUS       NP_415118               1293 aa            linear   CON 09-MAR-2022
            MG1655].
ACCESSION   NP_415118
VERSION     NP_415118.1
DBLINK      BioProject: PRJNA57779
            BioSample: SAMN02604091
DBSOURCE    REFSEQ: accession NC_000913.3
KEYWORDS    RefSeq.
SOURCE      Escherichia coli str. K-12 substr. MG1655
  ORGANISM  Escherichia coli str. K-12 substr. MG1655
            Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria;
            Enterobacterales; Enterobacteriaceae; Escherichia.
REFERENCE   1  (residues 1 to 1293)
  AUTHORS   Riley,M., Abe,T., Arnaud,M.B., Berlyn,M.K., Blattner,F.R.,
            Chaudhuri,R.R., Glasner,J.D., Horiuchi,T., Keseler,I.M., Kosuge,T.,
            Mori,H., Perna,N.T., Plunkett,G. III, Rudd,K.E., Serres,M.H.,
            Thomas,G.H., Thomson,N.R., Wishart,D. and Wanner,B.L.
  TITLE     Escherichia coli K-12: a cooperatively developed annotation
            snapshot--2005
  JOURNAL   Nucleic Acids Res. 34 (1), 1-9 (2006)
   PUBMED   16397293
  REMARK    Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1293)
  AUTHORS   Hayashi,K., Morooka,N., Yamamoto,Y., Fujita,K., Isono,K., Choi,S.,
            Ohtsubo,E., Baba,T., Wanner,B.L., Mori,H. and Horiuchi,T.
  TITLE     Highly accurate genome sequences of Escherichia coli K-12 strains
            MG1655 and W3110
  JOURNAL   Mol. Syst. Biol. 2, 2006 (2006)
   PUBMED   16738553
REFERENCE   3  (residues 1 to 1293)
  AUTHORS   Blattner,F.R., Plunkett,G. III, Bloch,C.A., Perna,N.T., Burland,V.,
            Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F.,
            Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J.,
            Mau,B. and Shao,Y.
  TITLE     The complete genome sequence of Escherichia coli K-12
  JOURNAL   Science 277 (5331), 1453-1462 (1997)
   PUBMED   9278503
REFERENCE   4  (residues 1 to 1293)
  AUTHORS   Arnaud,M., Berlyn,M.K.B., Blattner,F.R., Galperin,M.Y.,
            Glasner,J.D., Horiuchi,T., Kosuge,T., Mori,H., Perna,N.T.,
            Plunkett,G. III, Riley,M., Rudd,K.E., Serres,M.H., Thomas,G.H. and
            Wanner,B.L.
  TITLE     Workshop on Annotation of Escherichia coli K-12
  JOURNAL   Unpublished
  REMARK    Woods Hole, Mass., on 14-18 November 2003 (sequence corrections)
REFERENCE   5  (residues 1 to 1293)
  AUTHORS   Glasner,J.D., Perna,N.T., Plunkett,G. III, Anderson,B.D.,
            Bockhorst,J., Hu,J.C., Riley,M., Rudd,K.E. and Serres,M.H.
  TITLE     ASAP: Escherichia coli K-12 strain MG1655 version m56
  JOURNAL   Unpublished
  REMARK    ASAP download 10 June 2004 (annotation updates)
REFERENCE   6  (residues 1 to 1293)
  AUTHORS   Hayashi,K., Morooka,N., Mori,H. and Horiuchi,T.
  TITLE     A more accurate sequence comparison between genomes of Escherichia
            coli K12 W3110 and MG1655 strains
  JOURNAL   Unpublished
  REMARK    GenBank accessions AG613214 to AG613378 (sequence corrections)
REFERENCE   7  (residues 1 to 1293)
  AUTHORS   Perna,N.T.
  TITLE     Escherichia coli K-12 MG1655 yqiK-rfaE intergenic region, genomic
            sequence correction
  JOURNAL   Unpublished
  REMARK    GenBank accession AY605712 (sequence corrections)
REFERENCE   8  (residues 1 to 1293)
  AUTHORS   Rudd,K.E.
  TITLE     A manual approach to accurate translation start site annotation: an
            E. coli K-12 case study
  JOURNAL   Unpublished
REFERENCE   9  (residues 1 to 1293)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAR-2022) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   10 (residues 1 to 1293)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (30-JUL-2014) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Protein update by submitter
REFERENCE   11 (residues 1 to 1293)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-NOV-2013) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Protein update by submitter
REFERENCE   12 (residues 1 to 1293)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-SEP-2013) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Sequence update by submitter
REFERENCE   13 (residues 1 to 1293)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (06-FEB-2013) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Sequence update by submitter
REFERENCE   14 (residues 1 to 1293)
  AUTHORS   Rudd,K.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (24-APR-2007) Department of Biochemistry and Molecular
            Biology, University of Miami Miller School of Medicine, 118 Gautier
            Bldg., Miami, FL 33136, USA
  REMARK    Annotation update from ecogene.org as a multi-database
            collaboration
REFERENCE   15 (residues 1 to 1293)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-FEB-2006) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Protein updates by submitter
REFERENCE   16 (residues 1 to 1293)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2004) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
  REMARK    Sequence update by submitter
REFERENCE   17 (residues 1 to 1293)
  AUTHORS   Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-OCT-1998) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   18 (residues 1 to 1293)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
REFERENCE   19 (residues 1 to 1293)
  AUTHORS   Blattner,F.R. and Plunkett,G. III.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-JAN-1997) Laboratory of Genetics, University of
            Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence is identical to AAC73687.
            Changes to proteins and annotation made on March 7, 2022.  Current
            U00096 annotation updates are derived from EcoCyc
            https://ecocyc.org/.  Suggestions for updates can be sent to
            [email protected]. These updates are being generated from a
            collaboration  that includes EcoCyc, the University of Wisconsin,
            UniProtKB/Swiss-Prot, and the National Center for Biotechnology
            Information (NCBI).
            COMPLETENESS: full length.
            Method: conceptual translation.
FEATURES             Location/Qualifiers
     source          1..1293
                     /organism="Escherichia coli str. K-12 substr. MG1655"
                     /strain="K-12"
                     /sub_strain="MG1655"
                     /db_xref="taxon:511145"
     Protein         1..1293
                     /product="apo-serine activating enzyme"
                     /EC_number="6.3.2.14"
                     /calculated_mol_wt=141860
     Region          1..1292
                     /region_name="entF"
                     /note="enterobactin non-ribosomal peptide synthetase EntF;
                     PRK10252"
                     /db_xref="CDD:236668"
     CDS             1..1293
                     /gene="entF"
                     /locus_tag="b0586"
                     /gene_synonym="ECK0579"
                     /coded_by="NC_000913.3:614157..618038"
                     /transl_table=11
                     /db_xref="UniProtKB/Swiss-Prot:P11454"
                     /db_xref="ASAP:ABE-0002021"
                     /db_xref="ECOCYC:EG10264"
                     /db_xref="GeneID:945184"
CONTIG      join(WP_000077805.1:1..1293)
ORIGIN      
        1 msqhlplvaa qpgiwmaekl selpsawsva hyveltgevd spllaravva glaqadtlrm
       61 rftedngevw qwvddaltfe lpeiidlrtn idphgtaqal mqadlqqdlr vdsgkplvfh
      121 qliqvadnrw ywyqryhhll vdgfsfpait rqianiyctw lrgeptpasp ftpfadvvee
      181 yqqyreseaw qrdaafwaeq rrqlpppasl spaplpgrsa sadilrlkle ftdgefrqla
      241 tqlsgvqrtd lalalaalwl grlcnrmdya agfifmrrlg saaltatgpv lnvlplgihi
      301 aaqetlpela trlaaqlkkm rrhqrydaeq ivrdsgraag deplfgpvln ikvfdyqldi
      361 pdvqaqthtl atgpvndlel alfpdvhgdl sieilankqr ydeptliqha erlkmliaqf
      421 aadpallcgd vdimlpgeya qlaqlnatqv eipettlsal vaeqaaktpd apaladaryl
      481 fsyremreqv valanllrer gvkpgdsvav alprsvfltl alhaiveaga awlpldtgyp
      541 ddrlkmmled arpsllittd dqlprfsdvp nltslcynap ltpqgsaplq lsqphhtayi
      601 iftsgstgrp kgvmvgqtai vnrllwmqnh ypltgedvva qktpcsfdvs vweffwpfia
      661 gaklvmaepe ahrdplamqq ffaeygvttt hfvpsmlaaf vasltpqtar qscatlkqvf
      721 csgealpadl crewqqltga plhnlygpte aavdvswypa fgeelaqvrg ssvpigypvw
      781 ntglrildam mhpvppgvag dlyltgiqla qgylgrpdlt asrfiadpfa pgermyrtgd
      841 varwldngav eylgrsddql kirgqrielg eidrvmqalp dveqavthac vinqaaatgg
      901 darqlvgylv sqsglpldts alqaqlretl pphmvpvvll qlpqlplsan gkldrkalpl
      961 pelkaqapgr apkagsetii aaafssllgc dvqdadadff algghsllam klaaqlsrqv
     1021 arqvtpgqvm vastvaklat iidaeedstr rmgfetilpl regngptlfc fhpasgfawq
     1081 fsvlsryldp qwsiigiqsp rpngpmqtaa nldevceahl atlleqqphg pyyllgyslg
     1141 gtlaqgiaar lrargeqvaf lglldtwppe tqnwqekean gldpevlaei nrereaflaa
     1201 qqgststelf ttiegnyada vrllttahsv pfdgkatlfv aertlqegms perawspwia
     1261 eldiyrqdca hvdiispgtf ekigpiirat lnr