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LOCUS NP_414998 1120 aa linear CON 09-MAR-2022 [Escherichia coli str. K-12 substr. MG1655]. ACCESSION NP_414998 VERSION NP_414998.1 DBLINK BioProject: PRJNA57779 BioSample: SAMN02604091 DBSOURCE REFSEQ: accession NC_000913.3 KEYWORDS RefSeq. SOURCE Escherichia coli str. K-12 substr. MG1655 ORGANISM Escherichia coli str. K-12 substr. MG1655 Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 (residues 1 to 1120) AUTHORS Riley,M., Abe,T., Arnaud,M.B., Berlyn,M.K., Blattner,F.R., Chaudhuri,R.R., Glasner,J.D., Horiuchi,T., Keseler,I.M., Kosuge,T., Mori,H., Perna,N.T., Plunkett,G. III, Rudd,K.E., Serres,M.H., Thomas,G.H., Thomson,N.R., Wishart,D. and Wanner,B.L. TITLE Escherichia coli K-12: a cooperatively developed annotation snapshot--2005 JOURNAL Nucleic Acids Res. 34 (1), 1-9 (2006) PUBMED 16397293 REMARK Publication Status: Online-Only REFERENCE 2 (residues 1 to 1120) AUTHORS Hayashi,K., Morooka,N., Yamamoto,Y., Fujita,K., Isono,K., Choi,S., Ohtsubo,E., Baba,T., Wanner,B.L., Mori,H. and Horiuchi,T. TITLE Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110 JOURNAL Mol. Syst. Biol. 2, 2006 (2006) PUBMED 16738553 REFERENCE 3 (residues 1 to 1120) AUTHORS Blattner,F.R., Plunkett,G. III, Bloch,C.A., Perna,N.T., Burland,V., Riley,M., Collado-Vides,J., Glasner,J.D., Rode,C.K., Mayhew,G.F., Gregor,J., Davis,N.W., Kirkpatrick,H.A., Goeden,M.A., Rose,D.J., Mau,B. and Shao,Y. TITLE The complete genome sequence of Escherichia coli K-12 JOURNAL Science 277 (5331), 1453-1462 (1997) PUBMED 9278503 REFERENCE 4 (residues 1 to 1120) AUTHORS Arnaud,M., Berlyn,M.K.B., Blattner,F.R., Galperin,M.Y., Glasner,J.D., Horiuchi,T., Kosuge,T., Mori,H., Perna,N.T., Plunkett,G. III, Riley,M., Rudd,K.E., Serres,M.H., Thomas,G.H. and Wanner,B.L. TITLE Workshop on Annotation of Escherichia coli K-12 JOURNAL Unpublished REMARK Woods Hole, Mass., on 14-18 November 2003 (sequence corrections) REFERENCE 5 (residues 1 to 1120) AUTHORS Glasner,J.D., Perna,N.T., Plunkett,G. III, Anderson,B.D., Bockhorst,J., Hu,J.C., Riley,M., Rudd,K.E. and Serres,M.H. TITLE ASAP: Escherichia coli K-12 strain MG1655 version m56 JOURNAL Unpublished REMARK ASAP download 10 June 2004 (annotation updates) REFERENCE 6 (residues 1 to 1120) AUTHORS Hayashi,K., Morooka,N., Mori,H. and Horiuchi,T. TITLE A more accurate sequence comparison between genomes of Escherichia coli K12 W3110 and MG1655 strains JOURNAL Unpublished REMARK GenBank accessions AG613214 to AG613378 (sequence corrections) REFERENCE 7 (residues 1 to 1120) AUTHORS Perna,N.T. TITLE Escherichia coli K-12 MG1655 yqiK-rfaE intergenic region, genomic sequence correction JOURNAL Unpublished REMARK GenBank accession AY605712 (sequence corrections) REFERENCE 8 (residues 1 to 1120) AUTHORS Rudd,K.E. TITLE A manual approach to accurate translation start site annotation: an E. coli K-12 case study JOURNAL Unpublished REFERENCE 9 (residues 1 to 1120) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (08-MAR-2022) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 10 (residues 1 to 1120) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (30-JUL-2014) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Protein update by submitter REFERENCE 11 (residues 1 to 1120) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (15-NOV-2013) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Protein update by submitter REFERENCE 12 (residues 1 to 1120) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (26-SEP-2013) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Sequence update by submitter REFERENCE 13 (residues 1 to 1120) AUTHORS Rudd,K.E. TITLE Direct Submission JOURNAL Submitted (06-FEB-2013) Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, 118 Gautier Bldg., Miami, FL 33136, USA REMARK Sequence update by submitter REFERENCE 14 (residues 1 to 1120) AUTHORS Rudd,K.E. TITLE Direct Submission JOURNAL Submitted (24-APR-2007) Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine, 118 Gautier Bldg., Miami, FL 33136, USA REMARK Annotation update from ecogene.org as a multi-database collaboration REFERENCE 15 (residues 1 to 1120) AUTHORS Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (07-FEB-2006) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Protein updates by submitter REFERENCE 16 (residues 1 to 1120) AUTHORS Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (10-JUN-2004) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REMARK Sequence update by submitter REFERENCE 17 (residues 1 to 1120) AUTHORS Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (13-OCT-1998) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REFERENCE 18 (residues 1 to 1120) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (02-SEP-1997) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA REFERENCE 19 (residues 1 to 1120) AUTHORS Blattner,F.R. and Plunkett,G. III. TITLE Direct Submission JOURNAL Submitted (16-JAN-1997) Laboratory of Genetics, University of Wisconsin, 425G Henry Mall, Madison, WI 53706-1580, USA COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to AAC73567. Changes to proteins and annotation made on March 7, 2022. Current U00096 annotation updates are derived from EcoCyc https://ecocyc.org/. Suggestions for updates can be sent to [email protected]. These updates are being generated from a collaboration that includes EcoCyc, the University of Wisconsin, UniProtKB/Swiss-Prot, and the National Center for Biotechnology Information (NCBI). COMPLETENESS: full length. Method: conceptual translation. FEATURES Location/Qualifiers source 1..1120 /organism="Escherichia coli str. K-12 substr. MG1655" /strain="K-12" /sub_strain="MG1655" /db_xref="taxon:511145" Protein 1..1120 /product="potassium dependent, small conductance mechanosensitive channel" /calculated_mol_wt=127085 Region 7..1120 /region_name="PRK11281" /note="mechanosensitive channel MscK" /db_xref="CDD:236892" CDS 1..1120 /gene="mscK" /locus_tag="b0465" /gene_synonym="aefA; ECK0459; kefA" /coded_by="NC_000913.3:486536..489898" /transl_table=11 /db_xref="UniProtKB/Swiss-Prot:P77338" /db_xref="ASAP:ABE-0001613" /db_xref="ECOCYC:G6255" /db_xref="GeneID:945132" CONTIG join(WP_000177732.1:1..1120) ORIGIN 1 mtmfqyykrs rhfvfsafia fvfvllcqnt afarassngd lptkadlqaq ldslnkqkdl 61 saqdklvqqd ltdtlatldk idrikeetvq lrqkvaeape kmrqataalt alsdvdndee 121 trkilstlsl rqletrvaqa lddlqnaqnd lasynsqlvs lqtqpervqn amynasqqlq 181 qirsrldgtd vgetalrpsq kvlmqaqqal lnaeidqqrk slegntvlqd tlqkqrdyvt 241 ansarlehql qllqeavnsk rltltektaq eavspdeaar iqanplvkqe leinqqlsqr 301 litatengnq lmqqnikvkn wleralqser nikeqiavlk gslllsrily qqqqtlpsad 361 elenmtnria dlrleqfevn qqrdalfqsd afvnkleegh tnevnsevhd allqvvdmrr 421 elldqlnkql gnqlmmainl qinqqqlmsv sknlksiltq qifwvnsnrp mdwdwikafp 481 qslkdefksm kitvnwqkaw pavfiaflag lpllliagli hwrlgwlkay qqklasavgs 541 lrndsqlntp kailidlira lpvcliilav glilltmqln isellwsfsk klaifwlvfg 601 lcwkvlekng vavrhfgmpe qqtshwrrqi vrislallpi hfwsvvaels plhlmddvlg 661 qamiffnlll iaflvwpmcr eswrdkesht mrlvtitvls iipialmvlt atgyfyttlr 721 lagrwietvy lviiwnllyq tvlrglsvaa rriawrrala rrqnlvkega egaeppeept 781 ialeqvnqqt lritmllmfa lfgvmfwaiw sdlitvfsyl dsitlwhyng teagaavvkn 841 vtmgsllfai iasmvawali rnlpgllevl vlsrlnmrqg asyaittiln yiiiavgamt 901 vfgslgvswd klqwlaaals vglgfglqei fgnfvsglii lferpvrigd tvtigsfsgt 961 vskiriratt itdfdrkevi ipnkafvter linwsltdtt trlvirlgva ygsdlekvrk 1021 vllkaatehp rvmhepmpev fftafgastl dhelrlyvre lrdrsrtvde lnrtidqlcr 1081 endiniafnq levhlhnekg devtevkrdy kgddptpavg