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LOCUS NP_312586 934 aa linear CON 12-FEB-2021 ACCESSION NP_312586 VERSION NP_312586.1 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 934) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 934) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB37982. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..934 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..934 /product="T3SS intimin" /calculated_mol_wt=101705 Region 65..113 /region_name="LysM" /note="LysM domain; pfam01476" /db_xref="CDD:396179" Region 159..852 /region_name="Ve_IatC_Nterm" /note="inverse autotransporter adhesin IatC N-terminal domain; NF040978" /db_xref="CDD:468908" Region 171..442 /region_name="IAT_beta" /note="Inverse autotransporter, beta-domain; pfam11924" /db_xref="CDD:432192" Region 839..934 /region_name="Intimin_C" /note="Intimin C-type lectin domain; pfam07979" /db_xref="CDD:311782" CDS 1..934 /gene="eae" /locus_tag="ECs_4559" /coded_by="complement(NC_002695.2:4596583..4599387)" /transl_table=11 /db_xref="GeneID:915471" CONTIG join(WP_000627885.1:1..934) ORIGIN 1 mithgcytrt rhkhklkktl imlsaglglf fyvnqnsfan genyfklgsd skllthdsyq 61 nrlfytlktg etvadlsksq dinlstiwsl nkhlyssese mmkaapgqqi ilplkklpfe 121 ysalpllgsa plvaaggvag htnkltkmsp dvtksnmtdd kalnyaaqqa aslgsqlqsr 181 slngdyakdt algiagnqas sqlqawlqhy gtaevnlqsg nnfdgssldf llpfydsekm 241 lafgqvgary idsrftanlg agqrfflpan mlgynvfidq dfsgdntrlg iggeywrdyf 301 kssvngyfrm sgwhesynkk dyderpangf dirfngylps ypalgakliy eqyygdnval 361 fnsdklqsnp gaatvgvnyt piplvtmgid yrhgtgnend llysmqfryq fdkswsqqie 421 pqyvnelrtl sgsrydlvqr nnniileykk qdilslniph dingtehstq kiqlivksky 481 gldrivwdds alrsqggqiq hsgsqsaqdy qailpayvqg gsniykvtar aydrngnssn 541 nvqltitvls ngqvvdqvgv tdftadktsa kadnadtity tatvkkngva qanvpvsfni 601 vsgtatlgan saktdangka tvtlksstpg qvvvsaktae mtsalnasav iffdqtkasi 661 teikadktta vangkdaiky tvkvmkngqp vnnqsvtfst nfgmfngksq tqattgndgr 721 atitltsssa gkatvsatvs dgaevkatev tffdelkidn kvdiignnvr gelpniwlqy 781 gqfklkasgg dgtyswysen tsiatvdasg kvtlngkgsv vikatsgdkq tvsytikaps 841 ymikvdkqay yadamsickn llpstqtvls diydswgaan kyshyssmns itawikqtss 901 eqrsgvssty nlitqnplpg vnvntpnvya vcve