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LOCUS NP_312118 1517 aa linear CON 12-FEB-2021 Sakai]. ACCESSION NP_312118 VERSION NP_312118.3 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 1517) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 1517) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB37514. On Aug 15, 2018 this sequence version replaced NP_312118.2. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..1517 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..1517 /product="glutamate synthase large subunit" /calculated_mol_wt=166592 Region 30..1512 /region_name="gltB" /note="glutamate synthase subunit alpha; Provisional; PRK11750" /db_xref="CDD:236968" CDS 1..1517 /gene="gltB" /locus_tag="ECs_4091" /coded_by="NC_002695.2:4097112..4101665" /transl_table=11 /db_xref="GeneID:916060" CONTIG join(WP_001301585.1:1..1517) ORIGIN 1 mtrkprrhal svpvrsgsev gfpqslgevh dmlydksler dncgfgliah iegepshkvv 61 rtaihalarm qhrgailadg ktgdgcglll qkpdrffriv aqergwrlak nyavgmlfln 121 kdpelaaaar riveeelqre tlsivgwrdv ptnegvlgei alsslprieq ifvnapagwr 181 prdmerrlfi arrriekrle adkdfyvcsl snlvniykgl cmpadlprfy ldladlrles 241 aiclfhqrfs tntvprwpla qpfrylahng eintitgnrq warartykfq tplipdlhda 301 apfvneigsd sssmdnmlel llaggmdiir amrllvppaw qnnpdmdpel raffdfnsmh 361 mepwdgpagi vmsdgrfaac nldrnglrpa ryvitkdkli tcasevgiwd yqpdevvekg 421 rvgpgelmvi dtrsgrilhs aetdddlksr hpykewmekn vrrlvpfedl pdeevgsrel 481 dddtlasyqk qfnysaeeld svirvlgeng qeavgsmgdd tpfavlssqp riiydyfrqq 541 faqvtnppid plreahvmsl atsigremnv fceaegqahr lsfkspilly sdfkqlttmk 601 eehyradtld itfdvtkttl eatvkelcdk aekmvrsgtv llvlsdrnia kdrlpvpapm 661 avgaiqtrlv dqslrcdani ivetasardp hhfavllgfg ataiypylay etlgrlvdth 721 aiakdyrtvm lnyrnginkg lykimskmgi stiasyrcsk lfeavglhnd vvglcfqgav 781 sriggasfed fqqdllnlsk rawlarkpis qggllkyvhg geyhaynpdv vrtlqqavqs 841 geysdyqeya klvnerpatt lrdllaitpg enavniadve paselfkrfd taamsigals 901 peahealaea mnsiggnsns geggedpary gtnkvsrikq vasgrfgvtp aylvnadviq 961 ikvaqgakpg eggqlpgdkv tpyiaklrys vpgvtlispp phhdiysied laqlifdlkq 1021 vnpkamisvk lvsepgvgti atgvakayad litiagydgg tgasplssvk yagcpwelgl 1081 vetqqalvan glrhkirlqv dgglktgvdi ikaailgaes fgfgtgpmva lgckylrich 1141 lnncatgvat qddklrknhy hglpfkvtny fefiaretre lmaqlgvtrl vdligrtdll 1201 keldgftakq qklalsklle taephpgkal yctetnppfd ngllnaqllq qakpfvderq 1261 sktfwfdirn tdrsvgasls gyiaqthgdq glaadpikay fngtagqsfg vwnaggvely 1321 ltgdandyvg kgmaggliai rppvgsafrs heasiigntc lygatggrly aagragerfg 1381 vrnsgaitvv egigdngcey mtggivcilg ktgvnfgagm tggfayvlde sgdfrkrvnp 1441 elvevlsvdd laiheehlrg litehvqhtg sqrgeeilan wstfatkfal vkpkssdvka 1501 llghrsrsaa elrvqaq