Unfortunately due to lack of commercial feasibility, the SkyBLAST service has been suspended from December 1st, 2025.
All subscriptions for paid accounts have been paused. For further information or enquiries, please email [email protected]
LOCUS NP_311450 1295 aa linear CON 12-FEB-2021 O157:H7 str. Sakai]. ACCESSION NP_311450 VERSION NP_311450.1 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 1295) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 1295) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB36846. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..1295 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..1295 /product="phosphoribosylformyl-glycineamide synthetase" /calculated_mol_wt=141270 Region 2..1295 /region_name="PRK05297" /note="phosphoribosylformylglycinamidine synthase; Provisional" /db_xref="CDD:235394" CDS 1..1295 /gene="purL" /locus_tag="ECs_3423" /coded_by="complement(NC_002695.2:3412175..3416062)" /transl_table=11 /db_xref="GeneID:914912" CONTIG join(WP_000970087.1:1..1295) ORIGIN 1 mmeilrgspa lsafrinkll arfqaarlpv hniyaeyvhf adlnaplndd ehaqlerllk 61 ygpalashap qgklllvtpr pgtispwssk atdiahncgl qqvnrlergv ayyieagtlt 121 neqwqqvtae lhdrmmetvf falddaeqlf ahhqptpvts vdllgqgrqa lidanlrlgl 181 alaedeidyl qdaftklgrn pndielymfa qansehcrhk ifnadwiidg eqqpkslfkm 241 ikntfettpd hvlsaykdna avmegsevgr yfadhetgry dfhqepahil mkvethnhpt 301 aispwpgaat gsggeirdeg atgrgakpka glvgfsvsnl ripgfeqpwe edfgkperiv 361 taldimtegp lggaafnnef grpalngyfr tyeekvnshn geelrgyhkp imlaggigni 421 radhvqkgei nvgaklvvlg gpamniglgg gaassmasgq sdadldfasv qrdnpemerr 481 cqevidrcwq lgdanpilfi hdvgagglsn ampelvsdgg rggkfelrdi lsdepgmspl 541 eiwcnesqer yvlavaadql plfdelckre rapyavigea teelhlslhd rhfdnqpidl 601 pldvllgktp kmtrdvqtlk akgdalvreg itiadavkrv lhlptvaekt flvtigdrsv 661 tgmvardqmv gpwqvpvanc avttasldsy ygeamaiger apvalldfaa sarlavgeal 721 tniaatqigd ikriklsanw maaaghpged aglyeavkav geelcpalgl tipvgkdsms 781 mktrwqegne eremtsplsl visafarved vrhtitpqls tednalllid lgkgnnalga 841 talaqvyrql gdkpadvrdv aqlkgfydai qalvaqrkll ayhdrsdggl lvtlaemafa 901 ghcgidadia tlgddrlaal fneelgaviq vraadreave svlaqhglad cvhyvgqavs 961 gdrfvitang qtvfsesrtt lrvwwaettw qmqrlrdnpe cadqehqaks ndadpglnvk 1021 lsfdinedva apyiatgarp kvavlreqgv nshvemaaaf hragfdaidv hmsdllagrt 1081 gledfhalva cggfsygdvl gagegwaksi lfndrvrdef atffhrpqtl algvcngcqm 1141 msnlrelipg selwprfvrn tsdrfearfs lvevtqspsl llqgmvgsqm piavshgegr 1201 vevrdaahla aleskglval ryvdnfgkvt etypanpngs pngitavtte sgrvtimmph 1261 pervfrtvsn swhpenwged gpwmrifrna rkqlg