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LOCUS NP_310105 1015 aa linear CON 12-FEB-2021 Sakai]. ACCESSION NP_310105 VERSION NP_310105.1 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 1015) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 1015) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB35501. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..1015 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..1015 /product="formate dehydrogenase subunit alpha" /calculated_mol_wt=112793 Region 2..1014 /region_name="Molybdopterin-Binding" /note="Molybdopterin-Binding (MopB) domain of the MopB superfamily of proteins, a large, diverse, heterogeneous superfamily of enzymes that, in general, bind molybdopterin as a cofactor. The MopB domain is found in a wide variety of molybdenum- and...; cl09928" /db_xref="CDD:447860" Site order(50,52..53,57,92,238..239) /site_type="other" /note="[4Fe-4S] binding site [ion binding]" /db_xref="CDD:239153" Site order(94,192,196,230,232,235..236,259..261,278,280, 409..411,414..415,447..448,556..558,562,582..584,587,617, 649) /site_type="other" /note="molybdopterin cofactor binding site" /db_xref="CDD:239153" CDS 1..1015 /gene="fdnG" /locus_tag="ECs_2078" /coded_by="NC_002695.2:2068960..2072007" /note="codon on position 196 is selenocysteine opalcodon" /transl_table=11 /db_xref="GeneID:917275" CONTIG join(WP_010904702.1:1..1015) ORIGIN 1 mdvsrrqffk icaggmagtt vaalgfapkq alaqarnykl lrakeirntc tycsvgcgll 61 myslgdgakn areaiyhieg dpdhpvsrga lcpkgaglld yvnsenrlry peyrapgsdk 121 wqrisweeaf sriaklmkad rdanfiekne qgvtvnrwls tgmlcasgas netgmltqkf 181 arslgmlavd nqarvuhgpt vaslaptfgr gamtnhwvdi knanvvmvmg gnaaeahpvg 241 frwameaknn ndatlivvdp rftrtasvad iyapirsgtd itflsgvlry liennkinae 301 yvkhytnasl lvrddfafed glfsgydaek rqydksswny qfdengyakr detlthprcv 361 wnllkehvsr ytpdvvenic gtpkadflkv cevlastsap drtttflyal gwtqhtvgaq 421 nirtmamiql llgnmgmagg gvnalrghsn iqgltdlgll stslpgyltl psekqvdlqs 481 yleantpkat lagqvnywsn ypkffvslmk sfygdaaqke nnwgydwlpk wdqtydviky 541 fnmmdegkvt gyfcqgfnpv asfpdknkvv sclsklkymv vidplvtets tfwqnhgesn 601 dvdpasiqte vfrlpstcfa eedgsiansg rwlqwhwkgq dapgearndg eilagiyhhl 661 relyqaeggk gveplmkmsw nykqphepqs devakenngy aledlydang vliakkgqll 721 ssfahlrddg ttasscwiyt gswteqgnqm anrdnsdpsg lgntlgwawa wplnrrvlyn 781 rasadingkp wdpkrmliqw ngskwtgndi pdfgnaapgt ptgpfimqpe gmgrlfaink 841 maegpfpehy epietplgtn plhpnvvsnp vvrlyeqdal rmgkkeqfpy vgttyrlteh 901 fhtwtkhall naiaqpeqfv eisetlaaak ginngdrvtv sskrgfirav avvtrrlkpl 961 nvngqqvetv gipihwgfeg varkgyiant ltpnvgdans qtpeykaflv nieka