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LOCUS NP_309316 852 aa linear CON 12-FEB-2021 coli O157:H7 str. Sakai]. ACCESSION NP_309316 VERSION NP_309316.1 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 852) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 852) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB34712. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..852 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..852 /product="3-oxoacyl-[acyl-carrier-protein] synthase synthase" /calculated_mol_wt=92051 Region 6..398 /region_name="KAS_I_II" /note="Beta-ketoacyl-acyl carrier protein (ACP) synthase (KAS), type I and II. KASs are responsible for the elongation steps in fatty acid biosynthesis. KASIII catalyses the initial condensation and KAS I and II catalyze further elongation steps by Claisen...; cd00834" /db_xref="CDD:238430" Site order(109,116..117,121,123..124,139,143,147,153,155, 157..159,171,174..175,178,198..199,202..203,254..258,272, 390,392) /site_type="other" /note="dimer interface [polypeptide binding]" /db_xref="CDD:238430" Site order(162,294,330) /site_type="active" /db_xref="CDD:238430" Region 413..851 /region_name="FabB" /note="3-oxoacyl-(acyl-carrier-protein) synthase [Lipid transport and metabolism, Secondary metabolites biosynthesis, transport and catabolism]; COG0304" /db_xref="CDD:440073" CDS 1..852 /locus_tag="ECs_1289" /coded_by="NC_002695.2:1358880..1361438" /transl_table=11 /db_xref="GeneID:912824" CONTIG join(WP_000856330.1:1..852) ORIGIN 1 mktnrkrvvi tglgilssla eniqdfreal lnkkngvkds erfakwfena raaevlhdie 61 ysdlnedvid sldnaalway kvgkdalsqa glmdsrdtlk etgmivgvss agteaflplf 121 eqrmedfslr kalfsgafss ccssvstllg lqggvelvat actaspnavg mafdyiqngk 181 sktmlavgte piylptfagf yalnvmhpdt ctpfsgqsgm sigegagaiv leeyehavar 241 gatiygeils yatscdafhe tgpdprasga vqvmnkaman agvtpeqidy vnahgtgtea 301 ndrietlamk kvfanhdkll vsstksyfgh nigaagivel iaclatlpdn rvlptlnfsh 361 arpncdldyv pnefrdrkvn ifmknnyafg gnnccmilsm epasipvsty dskrvaisgv 421 gavsaightl nqvleniwaq dhhvelgsvt fpddtleeak ellavldesn qfaelfdeey 481 sfseatlpet esnfktfqvs gleprkhlrr fdqrkatrgg tfalialtea leqakrkikr 541 dgdelgmimg msrgpqetty kylqslkpdp rkvrtsefpg slmnaiptfc gisegikgyt 601 ttlatgensa lgaltygyei vrqnlqpqvl vggadeyfps mslymdavtq kihmtaeasd 661 yqiygkdpqg yvpgegacml lledpqlava rgaevlaevv gygkscsnty fdaslleeks 721 asmalaiera lthagisagd idlvcgtsng sddhsrieid aiyntfaaqn pavpvvnyna 781 cfgfvasaag llnlavitdc ikkqavpaip ntveffderv nfvrqsmkla lnhvllvgat 841 eggnyyafvi kg