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LOCUS NP_308632 1616 aa linear CON 12-FEB-2021 Sakai]. ACCESSION NP_308632 VERSION NP_308632.1 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 1616) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 1616) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB34028. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..1616 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..1616 /product="type IV secretion protein Rhs" /calculated_mol_wt=179093 Region <252..305 /region_name="PAAR_RHS" /note="proline-alanine-alanine-arginine (PAAR) domain, also containing C-terminal Rearrangement hotspot (Rhs) extensions; cd14742" /db_xref="CDD:269827" Region 393..454 /region_name="DUF6531" /note="Domain of unknown function (DUF6531); pfam20148" /db_xref="CDD:466309" Region 553..593 /region_name="YD_repeat_2x" /note="YD repeat (two copies); TIGR01643" /db_xref="CDD:273728" Region <607..1461 /region_name="RHS_core" /note="RHS element core protein; NF041261" /db_xref="CDD:469161" CDS 1..1616 /locus_tag="ECs_0605" /coded_by="complement(NC_002695.2:668129..672979)" /transl_table=11 /db_xref="GeneID:916963" CONTIG join(WP_001289110.1:1..1616) ORIGIN 1 msegpggpqg ataggtlamr mlsqramaas qmkraandka iaqmlaskks gppaarlgde 61 iqhksflgal agavlgaivt iaegclimaa catgpyalvl vpalmyasyk asdyveekqn 121 qleswinsfc dtdgaintgs knvkingkpa araavtlppp pppgaipevp qgepswgdia 181 tdllesaaek avplakawgn avitltdsna gfmdrvsaga sllfpagpvl mefatmvggr 241 geikkdvdfp eagedtalcd kenkppriaq gssnvfinnq paarkgdkle csaaivegsp 301 dvfiggeqvt yldiqpefpp wqrmilggit iasyllppag llgklgnlak lgklgnllgk 361 sgkllgaklg allgktgnsl ksiankvirw vtdpvdpvtg aycdertdft lgqtlplsft 421 rfhssvlplh gltgvgwsds wseyawvreq gnrvdvislg atlnfafdge sdtavnpyha 481 qyilrrrddy lelfdrdals srffydafpg mrlrhpvtdd tsddrlahsp adrmymlggm 541 sdtasnritf erdtqyritg vshtdgirlk ltyhasgylk aihrtdngiq tlatyeqdar 601 ldyhlfyeyd aadriirwsd ndqtwsrfty daqgrcvtvt gaegyynatl dygdgcttvt 661 dgkgihryyy dpdgnilree apdgstttye wdefhhllar hspagrvekf eynaahgqls 721 rytaadgadw qycydergll snitapagqt wtqqcdergl pvslvspqge etrlaytpqg 781 llsgifrqde rrlgieydhh nwpetltdvm grehhteysg hdlpvkmrgp ggqsvrlqwq 841 qhhklsgler agtgaegfry drhgnllayt dgngvvwtme ygpfdlpvar tdgeghrwqy 901 rydkdtlqlt evinpqgesy lyildncgrv teerdwggvv cryrydadgl ctarvnglee 961 tilysrdaag rlaevitpeg ktqyaydksg rltgifspdg tsqrtgyder grvnvttqgr 1021 raieyhypde htvircilpp ederdrhpdg sllkttyryn aageltevil pgdetltfsr 1081 deagrevlrh snrgfaceqg wnaagqpvsq raglfpaeat wggllpsllr eyrydsagnv 1141 sgvtsredyg rethreyrld rngqvtavta sgtglgygeg detygydscg ylkaqsagrh 1201 risgetdqya aghrlkqagn tqydydaagr mvsrtkhrdg yrpeterfrw dsrdqltgyr 1261 saqgeqweyr hdasgrrtek rcdrkkirft ylwdgdsiae ireyrddkly svrhlvfngf 1321 elisqqfsrv rqphpsvapq wvtrtnhavs dltgrplmlf nsegktvwrp gqtslwglal 1381 slpadtdypd prgerdpead pgllyagqwq daesglcynr fryyepetgm ylvsdplglq 1441 ggeqtyryvp npcgyidplg laicqlarwt kwgseqsnis dvlnslgnra lkyangdwik 1501 seaafnkyin minkrleltg skfrveiqpa ikngervpat tngpfkvngk wtsgthytgg 1561 skrldagiid itsptnqygl hpviegfdit lnktkpsavd iysdvfggid indfrl