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LOCUS NP_308213 1160 aa linear CON 12-FEB-2021 str. Sakai]. ACCESSION NP_308213 VERSION NP_308213.1 DBLINK BioProject: PRJNA226 BioSample: SAMN01911278 DBSOURCE REFSEQ: accession NC_002695.2 KEYWORDS RefSeq. SOURCE Escherichia coli O157:H7 str. Sakai ORGANISM Escherichia coli O157:H7 str. Sakai Bacteria; Pseudomonadati; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. REFERENCE 1 AUTHORS Hayashi,T., Makino,K., Ohnishi,M., Kurokawa,K., Ishii,K., Yokoyama,K., Han,C.G., Ohtsubo,E., Nakayama,K., Murata,T., Tanaka,M., Tobe,T., Iida,T., Takami,H., Honda,T., Sasakawa,C., Ogasawara,N., Yasunaga,T., Kuhara,S., Shiba,T., Hattori,M. and Shinagawa,H. TITLE Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and genomic comparison with a laboratory strain K-12 JOURNAL DNA Res. 8 (1), 11-22 (2001) PUBMED 11258796 REMARK Erratum:[DNA Res 2001 Apr 27;8(2):96] REFERENCE 2 AUTHORS Yokoyama,K., Makino,K., Kubota,Y., Watanabe,M., Kimura,S., Yutsudo,C.H., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Yoh,M., Iida,T., Ohnishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT1-Sakai carrying the Shiga toxin 1 genes of the enterohemorrhagic Escherichia coli O157:H7 strain derived from the Sakai outbreak JOURNAL Gene 258 (1-2), 127-139 (2000) PUBMED 11111050 REFERENCE 3 AUTHORS Ohnishi,M., Murata,T., Nakayama,K., Kuhara,S., Hattori,M., Kurokawa,K., Yasunaga,T., Yokoyama,K., Makino,K., Shinagawa,H. and Hayashi,T. TITLE Comparative analysis of the whole set of rRNA operons between an enterohemorrhagic Escherichia coli O157:H7 Sakai strain and an Escherichia coli K-12 strain MG1655 JOURNAL Syst. Appl. Microbiol. 23 (3), 315-324 (2000) PUBMED 11108008 REFERENCE 4 AUTHORS Makino,K., Yokoyama,K., Kubota,Y., Yutsudo,C.H., Kimura,S., Kurokawa,K., Ishii,K., Hattori,M., Tatsuno,I., Abe,H., Iida,T., Yamamoto,K., Onishi,M., Hayashi,T., Yasunaga,T., Honda,T., Sasakawa,C. and Shinagawa,H. TITLE Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O157:H7 derived from the Sakai outbreak JOURNAL Genes Genet. Syst. 74 (5), 227-239 (1999) PUBMED 10734605 REFERENCE 5 (residues 1 to 1160) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (15-AUG-2018) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 6 (residues 1 to 1160) AUTHORS Ogura,Y., Kurokawa,K., Hayashi,T. and Hattori,M. TITLE Direct Submission JOURNAL Submitted (26-JUN-2000) Contact:Yoshitoshi Ogura Kyushu University; 31-1 Maidashi Higashi-ku, Fukuoka, Fukuoka 812-8582, Japan COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence is identical to BAB33609. On May 10, 2018 this sequence version replased gi: 47118301 This update is obtained as follows: the Sakai genome was resequenced by illumina MiSeq and PacBio RS II sequencers and 78 sites of single base error, 11 sites of insertion (10 one-base insertions and a 141-base insertion) and 16 sites of deletion (14 one-base deletions, a three-base deletion and a six-base deletion) were corrected. Annotation was performed using DFAST auto-annotation system with manual curation. This work was done in collaboration with Tetsuya Hayashi, Makoto Ohnishi, Keisuke Nakayama (Miyazaki Medical College), Kozo Makino, Ken Kurokawa, Katsushi Yokoyama, Masashi Tanaka, Takeshi Honda, Teruo Yasunaga, Hideo Shinagawa (Osaka University), Takahiro Murata (Shinshu University), Chang-Gyun Han, Eiichi Ohtsubo, Toru Tobe, Chihiro Sasakawa (University of Tokyo), Hideto Takami (Japan Marine Science and Technology Center), Naotake Ogasawara (Nara Institute of Science and Technology), Satoru Kuhara (Kuyshu University), and supported by the Research for the Future Program of the Japan Society for the Promotion of Science. Method: conceptual translation. FEATURES Location/Qualifiers source 1..1160 /organism="Escherichia coli O157:H7 str. Sakai" /strain="Sakai" /sub_strain="RIMD 0509952" /serovar="O157:H7" /db_xref="taxon:386585" Protein 1..1160 /product="DNA polymerase III alpha subunit DnaE" /calculated_mol_wt=129769 Region 5..1151 /region_name="dnaE" /note="DNA polymerase III subunit alpha; Validated; PRK05673" /db_xref="CDD:235554" CDS 1..1160 /gene="dnaE" /locus_tag="ECs_0186" /coded_by="NC_002695.2:208470..211952" /transl_table=11 /db_xref="GeneID:913906" CONTIG join(WP_001294772.1:1..1160) ORIGIN 1 mseprfvhlr vhsdysmidg laktaplvkk aaalgmpala itdftnlcgl vkfygaghga 61 gikpivgadf nvqcdllgde lthltvlaan ntgyqnltll iskayqrgyg aagpiidrdw 121 lielneglil lsggrmgdvg rsllrgnsal vdecvafyee hfpdryflel irtgrpdees 181 ylhaavelae arglpvvatn dvrfidssdf daheirvaih dgftlddpkr prnyspqqym 241 rseeemcelf adipealant veiakrcnvt vrlgeyflpq fptgdmsted ylvkrakegl 301 eerlaflfpd eeervkrrpe yderletelq vinqmgfpgy flivmefiqw skdngvpvgp 361 grgsgagslv ayalkitdld plefdllfer flnpervsmp dfdvdfcmek rdqviehvad 421 mygrdavsqi itfgtmaaka virdvgrvlg hpygfvdris klippdpgmt lakafeaepq 481 lpeiyeadee vkalidmark legvtrnagk haggvviapt kitdfaplyc deegkhpvtq 541 fdksdveyag lvkfdflglr tltiinwale minkrrakng eppldiaaip lddkksfdml 601 qrsettavfq lesrgmkdli krlqpdcfed mialvalfrp gplqsgmvdn fidrkhgree 661 isypdvqwqh eslkpvlept ygiilyqeqv mqiaqvlsgy tlggadmlrr amgkkkpeem 721 akqrsvfaeg aeknginael amkifdlvek fagygfnksh saayalvsyq tlwlkahypa 781 efmaavmtad mdntekvvgl vdecwrmglk ilppdinsgl yhfhvnddge ivygigaikg 841 vgegpieaii earnkggyfr elfdlcartd tkklnrrvle klimsgafdr lgphraalmn 901 slgdalkaad qhakaeaigq admfgvlaee peqieqsyas cqpwpeqvvl dgeretlgly 961 ltghpinqyl keieryvggv rlkdmhpter gkvitaaglv vaarvmvtkr gnrigictld 1021 drsgrlevml ftdaldkyqh llekdriliv sgqvsfddfs gglkmtarev mdideareky 1081 arglaisltd rqiddqllnr lrqslephrs gtipvhlyyq radararlrf gatwrvspsd 1141 rllndlrgli gseqvelefd