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LOCUS NM_078504 2963 bp mRNA linear INV 26-DEC-2023 (Top3beta), mRNA. ACCESSION NM_078504 VERSION NM_078504.4 DBLINK BioProject: PRJNA164 BioSample: SAMN02803731 KEYWORDS RefSeq. SOURCE Drosophila melanogaster (fruit fly) ORGANISM Drosophila melanogaster Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora. REFERENCE 1 (bases 1 to 2963) AUTHORS Matthews,B.B., Dos Santos,G., Crosby,M.A., Emmert,D.B., St Pierre,S.E., Gramates,L.S., Zhou,P., Schroeder,A.J., Falls,K., Strelets,V., Russo,S.M. and Gelbart,W.M. CONSRTM FlyBase Consortium TITLE Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data JOURNAL G3 (Bethesda) 5 (8), 1721-1736 (2015) PUBMED 26109357 REMARK Publication Status: Online-Only REFERENCE 2 (bases 1 to 2963) AUTHORS Crosby,M.A., Gramates,L.S., Dos Santos,G., Matthews,B.B., St Pierre,S.E., Zhou,P., Schroeder,A.J., Falls,K., Emmert,D.B., Russo,S.M. and Gelbart,W.M. CONSRTM FlyBase Consortium TITLE Gene Model Annotations for Drosophila melanogaster: The Rule-Benders JOURNAL G3 (Bethesda) 5 (8), 1737-1749 (2015) PUBMED 26109356 REMARK Publication Status: Online-Only REFERENCE 3 (bases 1 to 2963) AUTHORS Hoskins,R.A., Carlson,J.W., Wan,K.H., Park,S., Mendez,I., Galle,S.E., Booth,B.W., Pfeiffer,B.D., George,R.A., Svirskas,R., Krzywinski,M., Schein,J., Accardo,M.C., Damia,E., Messina,G., Mendez-Lago,M., de Pablos,B., Demakova,O.V., Andreyeva,E.N., Boldyreva,L.V., Marra,M., Carvalho,A.B., Dimitri,P., Villasante,A., Zhimulev,I.F., Rubin,G.M., Karpen,G.H. and Celniker,S.E. TITLE The Release 6 reference sequence of the Drosophila melanogaster genome JOURNAL Genome Res 25 (3), 445-458 (2015) PUBMED 25589440 REFERENCE 4 (bases 1 to 2963) AUTHORS Hoskins,R.A., Carlson,J.W., Kennedy,C., Acevedo,D., Evans-Holm,M., Frise,E., Wan,K.H., Park,S., Mendez-Lago,M., Rossi,F., Villasante,A., Dimitri,P., Karpen,G.H. and Celniker,S.E. TITLE Sequence finishing and mapping of Drosophila melanogaster heterochromatin JOURNAL Science 316 (5831), 1625-1628 (2007) PUBMED 17569867 REFERENCE 5 (bases 1 to 2963) AUTHORS Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H. TITLE The Release 5.1 annotation of Drosophila melanogaster heterochromatin JOURNAL Science 316 (5831), 1586-1591 (2007) PUBMED 17569856 REMARK Erratum:[Science. 2007 Sep 7;317(5843):1325] REFERENCE 6 (bases 1 to 2963) AUTHORS Quesneville,H., Bergman,C.M., Andrieu,O., Autard,D., Nouaud,D., Ashburner,M. and Anxolabehere,D. TITLE Combined evidence annotation of transposable elements in genome sequences JOURNAL PLoS Comput Biol 1 (2), 166-175 (2005) PUBMED 16110336 REFERENCE 7 (bases 1 to 2963) AUTHORS Hoskins,R.A., Smith,C.D., Carlson,J.W., Carvalho,A.B., Halpern,A., Kaminker,J.S., Kennedy,C., Mungall,C.J., Sullivan,B.A., Sutton,G.G., Yasuhara,J.C., Wakimoto,B.T., Myers,E.W., Celniker,S.E., Rubin,G.M. and Karpen,G.H. TITLE Heterochromatic sequences in a Drosophila whole-genome shotgun assembly JOURNAL Genome Biol 3 (12), RESEARCH0085 (2002) PUBMED 12537574 REFERENCE 8 (bases 1 to 2963) AUTHORS Kaminker,J.S., Bergman,C.M., Kronmiller,B., Carlson,J., Svirskas,R., Patel,S., Frise,E., Wheeler,D.A., Lewis,S.E., Rubin,G.M., Ashburner,M. and Celniker,S.E. TITLE The transposable elements of the Drosophila melanogaster euchromatin: a genomics perspective JOURNAL Genome Biol 3 (12), RESEARCH0084 (2002) PUBMED 12537573 REFERENCE 9 (bases 1 to 2963) AUTHORS Misra,S., Crosby,M.A., Mungall,C.J., Matthews,B.B., Campbell,K.S., Hradecky,P., Huang,Y., Kaminker,J.S., Millburn,G.H., Prochnik,S.E., Smith,C.D., Tupy,J.L., Whitfied,E.J., Bayraktaroglu,L., Berman,B.P., Bettencourt,B.R., Celniker,S.E., de Grey,A.D., Drysdale,R.A., Harris,N.L., Richter,J., Russo,S., Schroeder,A.J., Shu,S.Q., Stapleton,M., Yamada,C., Ashburner,M., Gelbart,W.M., Rubin,G.M. and Lewis,S.E. TITLE Annotation of the Drosophila melanogaster euchromatic genome: a systematic review JOURNAL Genome Biol 3 (12), RESEARCH0083 (2002) PUBMED 12537572 REFERENCE 10 (bases 1 to 2963) AUTHORS Celniker,S.E., Wheeler,D.A., Kronmiller,B., Carlson,J.W., Halpern,A., Patel,S., Adams,M., Champe,M., Dugan,S.P., Frise,E., Hodgson,A., George,R.A., Hoskins,R.A., Laverty,T., Muzny,D.M., Nelson,C.R., Pacleb,J.M., Park,S., Pfeiffer,B.D., Richards,S., Sodergren,E.J., Svirskas,R., Tabor,P.E., Wan,K., Stapleton,M., Sutton,G.G., Venter,C., Weinstock,G., Scherer,S.E., Myers,E.W., Gibbs,R.A. and Rubin,G.M. TITLE Finishing a whole-genome shotgun: release 3 of the Drosophila melanogaster euchromatic genome sequence JOURNAL Genome Biol 3 (12), RESEARCH0079 (2002) PUBMED 12537568 REFERENCE 11 (bases 1 to 2963) AUTHORS Adams,M.D., Celniker,S.E., Holt,R.A., Evans,C.A., Gocayne,J.D., Amanatides,P.G., Scherer,S.E., Li,P.W., Hoskins,R.A., Galle,R.F., George,R.A., Lewis,S.E., Richards,S., Ashburner,M., Henderson,S.N., Sutton,G.G., Wortman,J.R., Yandell,M.D., Zhang,Q., Chen,L.X., Brandon,R.C., Rogers,Y.H., Blazej,R.G., Champe,M., Pfeiffer,B.D., Wan,K.H., Doyle,C., Baxter,E.G., Helt,G., Nelson,C.R., Gabor,G.L., Abril,J.F., Agbayani,A., An,H.J., Andrews-Pfannkoch,C., Baldwin,D., Ballew,R.M., Basu,A., Baxendale,J., Bayraktaroglu,L., Beasley,E.M., Beeson,K.Y., Benos,P.V., Berman,B.P., Bhandari,D., Bolshakov,S., Borkova,D., Botchan,M.R., Bouck,J., Brokstein,P., Brottier,P., Burtis,K.C., Busam,D.A., Butler,H., Cadieu,E., Center,A., Chandra,I., Cherry,J.M., Cawley,S., Dahlke,C., Davenport,L.B., Davies,P., de Pablos,B., Delcher,A., Deng,Z., Mays,A.D., Dew,I., Dietz,S.M., Dodson,K., Doup,L.E., Downes,M., Dugan-Rocha,S., Dunkov,B.C., Dunn,P., Durbin,K.J., Evangelista,C.C., Ferraz,C., Ferriera,S., Fleischmann,W., Fosler,C., Gabrielian,A.E., Garg,N.S., Gelbart,W.M., Glasser,K., Glodek,A., Gong,F., Gorrell,J.H., Gu,Z., Guan,P., Harris,M., Harris,N.L., Harvey,D., Heiman,T.J., Hernandez,J.R., Houck,J., Hostin,D., Houston,K.A., Howland,T.J., Wei,M.H., Ibegwam,C., Jalali,M., Kalush,F., Karpen,G.H., Ke,Z., Kennison,J.A., Ketchum,K.A., Kimmel,B.E., Kodira,C.D., Kraft,C., Kravitz,S., Kulp,D., Lai,Z., Lasko,P., Lei,Y., Levitsky,A.A., Li,J., Li,Z., Liang,Y., Lin,X., Liu,X., Mattei,B., McIntosh,T.C., McLeod,M.P., McPherson,D., Merkulov,G., Milshina,N.V., Mobarry,C., Morris,J., Moshrefi,A., Mount,S.M., Moy,M., Murphy,B., Murphy,L., Muzny,D.M., Nelson,D.L., Nelson,D.R., Nelson,K.A., Nixon,K., Nusskern,D.R., Pacleb,J.M., Palazzolo,M., Pittman,G.S., Pan,S., Pollard,J., Puri,V., Reese,M.G., Reinert,K., Remington,K., Saunders,R.D., Scheeler,F., Shen,H., Shue,B.C., Siden-Kiamos,I., Simpson,M., Skupski,M.P., Smith,T., Spier,E., Spradling,A.C., Stapleton,M., Strong,R., Sun,E., Svirskas,R., Tector,C., Turner,R., Venter,E., Wang,A.H., Wang,X., Wang,Z.Y., Wassarman,D.A., Weinstock,G.M., Weissenbach,J., Williams,S.M., WoodageT, Worley,K.C., Wu,D., Yang,S., Yao,Q.A., Ye,J., Yeh,R.F., Zaveri,J.S., Zhan,M., Zhang,G., Zhao,Q., Zheng,L., Zheng,X.H., Zhong,F.N., Zhong,W., Zhou,X., Zhu,S., Zhu,X., Smith,H.O., Gibbs,R.A., Myers,E.W., Rubin,G.M. and Venter,J.C. TITLE The genome sequence of Drosophila melanogaster JOURNAL Science 287 (5461), 2185-2195 (2000) PUBMED 10731132 REFERENCE 12 (bases 1 to 2963) AUTHORS Celniker,S., Carlson,J., Wan,K., Pfeiffer,B., Frise,E., George,R., Hoskins,R., Stapleton,M., Pacleb,J., Park,S., Svirskas,R., Smith,E., Yu,C. and Rubin,G. CONSRTM Berkeley Drosophila Genome Project TITLE Drosophila melanogaster release 4 sequence JOURNAL Unpublished REFERENCE 13 (bases 1 to 2963) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (20-DEC-2023) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 14 (bases 1 to 2963) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (13-DEC-2023) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 15 (bases 1 to 2963) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (19-OCT-2022) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 16 (bases 1 to 2963) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (20-APR-2020) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 17 (bases 1 to 2963) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (22-APR-2019) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 18 (bases 1 to 2963) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (24-MAY-2018) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 19 (bases 1 to 2963) CONSRTM FlyBase TITLE Direct Submission JOURNAL Submitted (07-DEC-2016) FlyBase, Harvard University, Biological Laboratories, 16 Divinity Ave, Cambridge, MA 02138, USA REFERENCE 20 (bases 1 to 2963) AUTHORS Celniker,S., Carlson,J., Kennedy,C., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Karpen,G. TITLE Direct Submission JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One #Cyclotron RoadOne Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA REMARK Direct Submission REFERENCE 21 (bases 1 to 2963) AUTHORS Celniker,S., Carlson,J., Wan,K., Frise,E., Hoskins,R., Park,S., Svirskas,R. and Rubin,G. TITLE Direct Submission JOURNAL Submitted (10-AUG-2006) Berkeley Drosophila Genome Project, Lawrence Berkeley National Laboratory, One Cyclotron Road, MS 64-121, Berkeley, CA 94720, USA REMARK Direct Submission REFERENCE 22 (bases 1 to 2963) AUTHORS Smith,C.D., Shu,S., Mungall,C.J. and Karpen,G.H. CONSRTM Drosophila Heterochromatin Genome Project TITLE Direct Submission JOURNAL Submitted (01-AUG-2006) Drosophila Heterochromatin Genome Project, Ernest Orlando Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Mailstop 64-121, Berkeley, CA 94720, USA REFERENCE 23 (bases 1 to 2963) AUTHORS Adams,M.D., Celniker,S.E., Gibbs,R.A., Rubin,G.M. and Venter,C.J. TITLE Direct Submission JOURNAL Submitted (21-MAR-2000) Celera Genomics, 45 West Gude Drive, Rockville, MD 20850, USA COMMENT REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NC_004354). On Jul 15, 2014 this sequence version replaced NM_078504.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.54 URL :: http://flybase.org ##Genome-Annotation-Data-END## FEATURES Location/Qualifiers source 1..2963 /organism="Drosophila melanogaster" /mol_type="mRNA" /db_xref="taxon:7227" /chromosome="X" /genotype="y[1]; Gr22b[1] Gr22d[1] cn[1] CG33964[R4.2] bw[1] sp[1]; LysC[1] MstProx[1] GstD5[1] Rh6[1]" gene 1..2963 /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="Topoisomerase 3beta" /map="5E5-5E5" /db_xref="FLYBASE:FBgn0026015" /db_xref="GeneID:31565" CDS 260..2887 /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /EC_number="5.6.2.1" /note="CG3458 gene product from transcript CG3458-RA; CG3458-PA; Top3beta-PA; Top 3beta; drosophila topoisomerase 3beta" /codon_start=1 /product="topoisomerase 3beta, isoform A" /protein_id="NP_511059.2" /db_xref="FLYBASE:FBpp0070891" /db_xref="GeneID:31565" /db_xref="FLYBASE:FBgn0026015" /translation="MKSVLMVAEKPSLAASLAGILSNGRCTAKRGTGNGCSTHEWTGN FRNEGSVHFRMTSVCGHVMSLDFNKKYNCWDKVDPIQLFGCATEKKETNPKQNMRKFL AHEARGCDYLVLWLDCDKEGENICFEVMDAVKHVINNVYSDQVTYRAHFSAITEKDIK KAMETLGHPNENEAKSVDARQELDLRIGCAFTRFQTKFFQDRYGDLDSSLISYGPCQT PTLGFCVKRHDDIQTFKPESFWHLQLLAGQPEVTLEWARGRVFKKDIAIMLLNRVKEH KKATVESVASKEAYKSKPQALNTVELMRICSSGLGIGPFQAMQIAERLYTQGYISYPR TETNQYPTNFDLPAVLHVLKPSADFGEEARSILGDIQTPRKGKDAGDHPPITPMKLGN RSDFDRDTWRVYEFICRHFMGTVSRDLKYRVTTAKLSVGMETFSCTASVLIDAGFTKV MTWSAFGKDEPQPPFVQGTQVAINDVRLIESQTGPPDYLTESELITLMEEHGIGTDAS IPVHINNICQRNYVHIENGRKLMPTTLGIVLVHGYQKIDPELVLPTMRTEVERMLTLI AQGSANFQDVLRHAIKIFKLKFMYFVKNIDSMDALFEVSFSPLAESGKAHSRCGKCRR YMKYIQTKPARLHCSHCDETYALPIGNVKVYREFKCPLDDFDLLAFSTGVKGRSYPFC PYCYNHPPFSDMPHLGGCNTCTNANCPHSLNTLGISSCVECPTGVLVLDCTLAPTWKL GCNRCDVIINCFKGATKITVEEAKCQECGAQQVNVVYKSDKSKFKDGSEEKSGCIFCS ADFSHLVEKHRAVASRPVRSGGGFRGGKAGRGGGGMGGAAFGSGGAVTAGGGPNAGGG VRGSRVAKDKMGQLASYFV" misc_feature 269..757 /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="The topoisomerase-primase (TORPIM) domain found in members of the type IA family of DNA topoisomerases (Topo IA) similar to topoisomerase III. Type IA DNA topoisomerases remove (relax) negative supercoils in the DNA by: cleaving one strand of the DNA...; Region: TOPRIM_TopoIA_TopoIII; cd03362" /db_xref="CDD:173782" misc_feature order(284..289,296..298,608..610,614..616,620..622) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="active site" /db_xref="CDD:173782" misc_feature order(305..307,611..613,617..619,638..643,647..652) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="putative interdomain interaction site [polypeptide binding]; other site" /db_xref="CDD:173782" misc_feature order(608..610,614..616) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="putative metal-binding site [ion binding]; other site" /db_xref="CDD:173782" misc_feature 617..619 /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="putative nucleotide binding site [chemical binding]; other site" /db_xref="CDD:173782" misc_feature 770..2023 /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="DNA Topoisomerase, subtype IA; DNA-binding, ATP-binding and catalytic domain of bacterial DNA topoisomerases I and III, and eukaryotic DNA topoisomerase III and eubacterial and archael reverse gyrases. Topoisomerases clevage single or double stranded DNA...; Region: TOP1Ac; cd00186" /db_xref="CDD:238110" misc_feature order(770..853,890..952) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="domain I [active]" /db_xref="CDD:238110" misc_feature order(797..802,809..811,821..823,833..835,1223..1225, 1235..1237,1259..1261,1778..1783,1790..1795,1799..1801, 1811..1816) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="DNA binding groove [nucleotide binding]" /db_xref="CDD:238110" misc_feature order(923..925,935..937,1889..1891) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="phosphate binding site [ion binding]; other site" /db_xref="CDD:238110" misc_feature order(965..988,1091..1135,1508..1552,1556..1612, 1679..1711) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="domain II [active]" /db_xref="CDD:238110" misc_feature order(1136..1156,1160..1327,1400..1426,1445..1507) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="domain III [active]" /db_xref="CDD:238110" misc_feature order(1169..1171,1181..1189,1403..1405,1463..1465, 1472..1474,1484..1486,1772..1774,1778..1780,1847..1849) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="nucleotide binding site [chemical binding]; other site" /db_xref="CDD:238110" misc_feature order(1253..1255,1259..1261,1403..1405) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="catalytic site [active]" /db_xref="CDD:238110" misc_feature order(1712..1834,1847..1891,1907..2023) /gene="Top3beta" /locus_tag="Dmel_CG3458" /gene_synonym="CG3458; Dmel\CG3458; dTop3beta; top3; Top3; TOP3; top3beta; top3[beta]; Topo IIIbeta; topo III[beta]" /note="domain IV [active]" /db_xref="CDD:238110" ORIGIN 1 ccgtattatt ggctggaaaa taatgcccgt gaaatttgtt aaatagaggg gattatacgg 61 atgtatagcg aggaagtcca attcatccat tagcccgtgt tcgtgtgcgt ttatgctgtg 121 cgtgtgcgtt tgtgtgagtg tgtgtgcagc acgaaaccgg cgaatcccat gcgagtgaac 181 gagtgggcag gaggaagcgc tgcaaaaatc tcatcatcaa ttatataggc cattggattg 241 cgggacttgg atttgggcca tgaagagcgt gctgatggtg gccgagaagc cctcgctggc 301 ggcctcgctg gcggggattc tctcaaacgg acgctgcacg gccaagcgtg gcaccggcaa 361 cggctgctcc acgcacgagt ggacgggcaa ctttcggaac gagggcagcg tccacttccg 421 gatgacttcc gtgtgcggtc atgtcatgtc gctggacttc aataagaagt acaactgctg 481 ggacaaggtg gatcccatcc agctcttcgg ttgtgccacc gaaaagaagg aaacgaatcc 541 caagcagaac atgcgcaaat ttctggccca cgaggcgcgc ggctgtgact atctggtgct 601 ctggctggat tgcgacaagg agggcgagaa catctgcttc gaggtgatgg acgccgtgaa 661 gcatgtcatc aacaatgtgt acagcgatca ggtgacatac cgtgcccact tctcggctat 721 cacggagaag gacatcaaga aagccatgga gacgttgggg catcccaacg agaacgaggc 781 caagtcggtg gacgcccggc aggaattgga cctgcgcatt ggctgcgcct ttacccgctt 841 tcaaacgaag ttctttcagg atcgctacgg tgacctggat tcgtcgctga tctcctacgg 901 tccctgccag acgcccacgc tcggtttctg cgtgaagcgg cacgacgaca tccagacctt 961 taagccggag agcttctggc atctgcagct cctcgccggc cagccggaag tcacgcttga 1021 atgggcgcgc ggtcgggtct tcaagaagga catcgccatc atgctgctga atcgcgtcaa 1081 ggagcacaag aaggccactg tggagagcgt ggccagcaag gaggcctaca agagcaaacc 1141 acaggcactg aacaccgtcg aactgatgcg tatctgcagc tccggactgg gcatcggacc 1201 cttccaggcg atgcagatag ctgagcgcct gtacacgcag ggctacatca gctatccgcg 1261 aacagagacc aatcagtatc cgactaactt cgatctgccg gccgtgctgc acgtgctgaa 1321 gccatcggcg gacttcggtg aggaggcgcg ctccattttg ggcgacattc agacgccgcg 1381 aaagggcaag gatgccggcg accatccacc catcacgcca atgaaactgg gcaaccgcag 1441 tgacttcgat cgcgacacct ggcgtgtcta cgagttcatc tgccggcact tcatgggcac 1501 ggtgtcgagg gatcttaagt accgcgtgac gacggcaaag ctgagtgttg gcatggagac 1561 attcagttgc acggccagtg tgctgatcga tgccggtttc accaaggtga tgacgtggtc 1621 ggccttcggc aaggacgaac cccagccgcc gttcgttcag ggcactcagg tggccatcaa 1681 cgatgtgcga cttatcgaaa gccagacagg accgccggat tatctcaccg aatcggagct 1741 aatcacactg atggaggagc acggcatcgg gacggatgcc tccataccgg tgcacatcaa 1801 caacatctgc cagcgcaact atgtgcacat tgagaacggt cgcaagctga tgccaacgac 1861 gctgggcatt gtgctggtcc acggctatca gaagatcgat ccggaactgg tgctgcccac 1921 catgcgaacg gaggtggagc gcatgctgac actgattgcc cagggatcgg ctaactttca 1981 ggatgttctg cgacacgcca tcaagatttt caagctgaag tttatgtact ttgtgaagaa 2041 catcgacagc atggacgcac tgtttgaggt atccttctcg ccgctggccg agtcgggcaa 2101 ggcgcactcg cgctgcggca agtgtaggcg ctacatgaag tacatacaga ccaaaccggc 2161 gagattgcac tgctctcatt gcgatgagac atacgccctg cccattggca atgtgaaggt 2221 gtatcgcgag ttcaagtgcc cgctggacga ctttgatctg ctggctttct ccactggcgt 2281 gaaggggcgt tcgtatccgt tctgcccgta ctgttacaac catccgccgt tcagcgacat 2341 gccccacttg gggggctgca acacctgcac gaacgccaac tgcccgcact cgctgaacac 2401 gctgggcatc tccagctgcg tggagtgccc caccggggtc ctggtgctcg attgtacgct 2461 tgcgccaacc tggaagctgg gctgcaatcg ctgcgacgtg atcatcaact gcttcaaggg 2521 agccaccaag atcacggtgg aagaggccaa gtgccaggag tgtggcgccc agcaggtgaa 2581 tgtggtctac aagtcggaca agagcaagtt caaggacgga agcgaggaga agagcggctg 2641 catcttctgc tccgcagact tctcgcactt ggtcgagaag catcgggcgg tggcatccag 2701 accagttcgc agtggcggcg gcttccgtgg cggcaaggca ggtcgaggcg gtggcggaat 2761 gggcggggcg gcttttggct ccggaggagc ggttacggct ggcggtggac ctaatgctgg 2821 gggcggggtg cgtggcagtc gagtggccaa ggataagatg ggacaactgg cgtcgtattt 2881 cgtttgagga cactgaattg catttagaag aacaataact aaacaagaaa aaaaaagcct 2941 aataaaaacg aaattgtata aat